人精氨酸/丝氨酸丰富剪接因子1在膀胱尿路上皮癌中表达及临床意义

SRSF1 expression and clinical significance in bladder urothelial carcinoma

目的探讨人精氨酸/丝氨酸丰富剪接因子1(SRSF1)在膀胱尿路上皮细胞癌的表达及其临床意义。方法应用免疫组化方法检测41例膀胱尿路上皮癌及10例正常膀胱尿路上皮中SRSF1的表达情况。结合临床资料以及分析SRSF1与病理分期,组织学分级和复发转移情况的关系。结果 SRSF1在膀胱尿路上皮细胞癌的阳性表达率为83(34/41),而对照组的阳性率为10.0%(1/10),两组间比较差异有统计学意义(P<0.01)。SRSF1的阳性表达与膀胱尿路上皮癌的初发和复发、是否转移显著相关(P<0.05);但在病理分期,组织学分级和肿瘤数量无显著差异。结论 SRSF1在膀胱尿路上皮细胞癌的表达明显上调,并且与膀胱尿路上皮癌的复发及转移密切相关,SRSF1可能通过与肿瘤基因相互作用,调控细胞周期及凋亡等多途径,参与肿瘤的发生及发展。SRSF1可用于膀胱上皮细胞癌早期诊断和复发、转移的一项重要指标。

Objective To investigate the expression of SRSF1 in Bladder urothelial cell carcinoma, and its clinical significance. Methods The expression of SRSF1 in was detected with streptavidin-peroxidase Immunohistochemistry method in the paraffin embed-ded sections of 41 Bladder urothelial cell carcinoma patients,and its relationship with pathological stages,histological grades,recur-rence,metastasis was discussed. Normal bladder tissues from 10 health people were used as control group. Results The positive rate of the expression of SRSF1 in Bladder urothelial cell carcinoma was 83（34/41）,which was significantly different from the positive rate in control group 10. 0%（1/10）. The expression of SRSF1 was related to the recurrence and metastasis,but had no significant differ-ence in pathological stages,histological grades and number. Conclusion The up-regulated expression of SRSF1 in Bladder urothelial cell carcinoma suggests that SRSF1 plays an important role in tumor occurrence and progress through inhibiting cell apoptosis. Positive expression of SRSF1 in Bladder urothelial cell carcinoma is associated with high risk of recurrence,and can be used to monitor early diagnosis,recurrence and metastasis.