恶喹酸与诺氟沙星对异育银鲫体内嗜水气单胞菌的抑菌效果比较

Comparative Bacteriostasis of Oxolinic Acid and Norfloxcin against Pathogenic Bacterium Aeromonas hydrophila in Allogyogenetics Silver Crucian Carp Carassius auratus gobelio

在水温（25±2）℃下,给体质量150~200g的异育银鲫分别口灌3种剂量（20、30、40mg/kg）的诺氟沙星和恶喹酸,结合这两种药物对嗜水气单胞菌AH10的体外药效学研究和对异育银鲫单次口灌不同剂量的诺氟沙星、恶喹酸的体内药代动力学,研究诺氟沙星、恶喹酸对异育银鲫体内嗜水气单胞菌AH10的抑菌效果。试验结果表明,恶喹酸和诺氟沙星对嗜水气单胞菌AH10的最小抑菌质量浓度分别为1μg/mL和0.5μg/mL。口灌上述3种剂量恶喹酸、诺氟沙星后,异育银鲫血浆中恶喹酸的最大药物质量浓度分别为4.1μg/mL、6.0μg/mL和8.89μg/mL;诺氟沙星的最大药物质量浓度分别为11.5μg/mL、15.1μg/mL和18.9μg/mL;恶喹酸的最大药物质量浓度/最小抑菌质量浓度分别为4.1、6.0和8.89;诺氟沙星的最大药物质量浓度/最小抑菌质量浓度分别为23、30.2和37.8;恶喹酸0~24h内药—时曲线下面积/最小抑菌质量浓度分别为21.6214、33.1449、39.1846;诺氟沙星0~24h内药—时曲线下面积/最小抑菌质量浓度分别为274.75、451.55、578.35。综合0~24h内药—时曲线下面积/最小抑菌质量浓度、最大药物质量浓度/最小抑菌质量浓度这两个指标可知,诺氟沙星对异育银鲫体内的嗜水气单胞菌AH10抑制效果强于恶喹酸。

In vitro and in vivo pharmacokinetics of oxolinic acid and norfloxcin against pathogenic bacteri- um Aeromonas hydrophila （AH10） were studied in allogyogenetics silver crucian carp Carassius auratus gobelio with body weight of 150 200 g singly and orally administrated at a dose of 20,30,and 40 mg per kg body weight at water temperature of （25-52） ℃. The minimal inhibitory concentration （MIC） on AH10 was shown to be 1 μg/mL in oxolinic acid and 0.5 μg/mL in norfloxcin. The maximum serum concentration of oxolinic acid in allogyogenetics silver crucian carp was 4. 1 μg/mL at a dose of 20 mg per kg body weight,6.0 μg/mL at a dose of 30 mg per kg body weight,and 8.89 μg/mL at a dose of 40 mg per kg body weight,and the maximum serum concentration of norfloxcin were 11.5 μg/mL at a dose of 20 mg per kg body weight,15.1 μg/mL at a dose of 30 mg per kg body weight, and 18.9 μg/mL at a dose of 40 mg per kg body weight. Cmax of oxolinic acid/MIC of oxolinic acid was 4.1,6.0 and 8.89 at various doses,respec tively; Cmax of norfloxcin/MIC of norfloxcin was 23,30.2 and 37.8 at various doses,respectively. The ar ea under the curve （AUC24,0 24 h） of oxolinic acid/MIC of oxolinic acid was 21. 6214, 33. 1449 and 39. 1846 at various doses, respectively; AUC24 of norfloxcin /MIC of norfloxcin was 274. 75,451. 55 and 578.35 at various doses,respectively. Based on the AUC24/MIC and Cmax/MIC,there was greater bacteri- ostasis in norfloxcin than in oxolinic acid against A. hydrophila in allogyogenetics silver crucian carp.