连续5d快速动眼睡眠剥夺诱发小鼠迟发性抑郁样行为

Rapid eye-movement sleep for five days deprivation causes delayed depressive-like behavior in mice

目的：探讨连续长时间快速动眼睡眠剥夺（5 d）对C57BL/6J小鼠抑郁样行为和杏仁核单胺氧化酶A水平的影响。方法成年雄性C57BL/6J小鼠随机分成空白组、对照组和快速动眼睡眠剥夺组，通过多小平台水环境法建立快速动眼睡眠剥夺模型。睡眠剥夺结束后，于不同时间点采用旷场实验检测各组运动能力，强迫游泳实验和糖水偏好实验检测各组抑郁样行为。之后，取脑分离杏仁核，利用Western Blot和Real-time PCR检测各组单胺氧化酶A的表达水平。结果快速动眼睡眠剥夺后的1~3 d内，睡眠剥夺小鼠运动能力明显下降，第4天可恢复到正常水平；第7~14天强迫游泳实验显示小鼠不动时间延长；糖水偏好率也明显降低。Western blot和Real-time PCR检测发现杏仁核中单胺氧化酶A表达水平明显升高。结论连续长时间快速动眼睡眠剥夺导致小鼠迟发性抑郁样行为，该现象很可能与杏仁核单胺氧化酶水平变化有关。

Objective To observe the effect of rapid eye movement sleep deprivation （REMSD） for 5 days on depressive-like behavior and monoamine oxidase A （MAOA） expression in the amygdale of mice. Methods Adult male C57BL/6J mice were divided into blank control group, control group and REMSD group. REMSD models were established by a modified multiple small platform method. The mice were examined for locomotive activity in open field test （OFT） and for depressive-like behavior in forced swimming test （FST） and sucrose preference test （SPT） after treatment. After all the tests, the protein and mRNA expressions of MAOA in the amygdala were detected with Western blotting and real-time PCR, respectively. Results REMSD for 5 days significantly impaired the locomotive activity of the mice, which was obvious in 1 to 3 days after REMSD. The locomotive activity became normal on day 4 after the 5-day REMSD. The immobility time of the mice was lengthened in days 7 to 14 （P〈0.01）, and sucrose preference rate was reduced significantly in days 8 and 9 （P〈0.01）. The expression level of MAOA in the amygdala was increased significantly after the 5-day REMSD （P〈0.01）. Conclusion REMSD for 5 days causes delayed depressive-like behavior in mice possible in relation with the increased expression of MAOA in the amygdale.