试管微环境对马铃薯‘GSAP-H’愈伤组织花青素含量的影响

Effect of microenvironment on the anthocyanin content from the callus of potato 'GSAP-H'

【目的】为获得愈伤组织生长及花青素积累的最佳培养条件.【方法】针对马铃薯"GSAP-H"试管苗茎叶诱导出的紫色愈伤组织进行了外源激素、糖源、不同N素比例、光温和pH等条件的研究.【结果】随NAA、2,4-D浓度的增加,愈伤组织快速生长,花青素含量呈先升后降的趋势,于1.5mg/L 2,4-D和1.0mg/L NAA浓度下达到高峰,分别为863.15、880.65nmol/g;高浓度BAP不利于愈伤组织的生长,但花青素含量在4mg/L BAP下达到高峰,为848.745nmol/g.蔗糖是花青素生产的最好碳源,45g/L蔗糖下的愈伤组织生长最快且花青素含量最高(887.035nmol/g);培养基中NO_3^-与NH_4^+比例为3:1时,愈伤组织花青素含量最高(895.54nmol/g).此外,全光照利于花青素产量的提高.pH 4.8及25℃的培养条件可明显促进愈伤组织花青素的积累.【结论】研究结果将为利用紫色马铃薯愈伤组织进行工厂化生产花青素奠定一定基础.

[Objective] In order to obtain the optimal condition for callus growth and anthocyanin accu- mulation. [Method] Potato purple callus was induced from the leaves and stems of ＇ GSAP-H＇ in vitro. The purple callus was treated by different level of exogenous hormones, sugar source, nitrogen source, illumina- tion time,cultural temperature and pH value. [Result] The results showed that callus grew rapidly with the increasing concentration of NAA and 2,4-D,anthocyanin content rose first then decreased and reached the maximum under 1.5 mg/L 2,4-D and 1.0 mg/L NAA,by 863. 15 nmol/g and 880.65 nmol/g,respec- tively. High concentration of BAP was against callus growth, but the anthocyanin content reached the peak under 4 mg/L BAP concentration level （848.75 nmol/g）. Sucrose was the best carbon source for anthocya- highest （887. 04 nmol/g） after the purple callus was cultured in medium with 45 g/L sucrose. With the rario of NO3- to NH4＋ reaching 3：1, the anthocyanin content also rose up to the highest （895.54 nmol/g. FW）. In addition,the culturing condition of 25℃ and full exposure could obviously promote the anthocya nin accumulation of purple callus growing in medium with pH 4. 8. [Conclusion] The results could be ap plicable for industrializing production of anthocyanin with purple potato callus in the future.