R(+)普拉克索对脑缺血再灌注大鼠早期脑损伤保护作用

PROTECTION OF R+PRAMIPEXOLE ON EARLY BRAIN INJURY IN RATS FOLLOWING CEREBRAL ISCHEMIA-REPERFUSION

目的研究R(+)普拉克索(R+PPX)对脑缺血再灌注(CIR)大鼠早期活性氧(ROS)生成的影响,探讨R+PPX对CIR早期大鼠脑组织的保护作用,为缺血性脑卒中的有效治疗提供实验依据。方法采用线栓法建立大鼠CIR模型,缺血时间2h后,采用抽签方法将100只健康雄性大鼠分为假手术组(A组,n=20)、CIR组(B组,n=30)、R+PPX注射CIR组(C组,n=50)3组。于再灌注8和24h时,行TTC染色,LOZEX-F扫描计算脑梗死体积百分比;采用二氯荧光素(H2DCF-DA)探针监测大鼠脑内ROS阳性细胞数目和荧光强度。结果制作CIR模型的过程中,A、B、C 3组大鼠的肛温、心率、动脉压指标差异无显著性(P>0.05)。A组未出现脑梗死,再灌注8h时,C组脑梗死体积明显小于B组(F=15.32,P<0.05),但再灌注24h时,B组脑梗死体积百分比与C组比较,差异无显著意义(P>0.05)。再灌注8h时,C组ROS阳性细胞数明显少于B组(F=25.72,P<0.05),但再灌注24h时,B、C两组的ROS阳性细胞数差异无显著性(P>0.05)。结论 R+PPX预处理可以明显减少CIR早期大鼠体内的ROS生成水平及脑梗死体积,从而对CIR早期脑损伤起到保护作用。

Objective To study the effects of R（--） pramipexole （R＋PPX） on the generation of reactive oxygen species （ROS） in early cerebral ischemia reperfusion （CIR） in rats, discuss the protective effect of R-＋-PPX on early CIR rats, and provide experimental evidence for effective treatment of cerebral ischemic stroke （CIS）. Methods A CIR model in rats was created using thread bolt method. After 2 h of ischemia, 100 healthy male rats were divided into three groups by draw method： sham operation group （group A, n^20）; CIR group （group B, n：30）; R--PPX injection CIR group （group C, n =50）. At 8 h and 24 h after reperfusion, the volume of cerebral infarction was determined using TTC and LOZEX-F image scanning, the number of positive ROS cells in rat brain and fluorescence intensity were monitored applying fluorescent probe H2DCF-DA. Results In the process of making CIR model, the differences in rectal temperature, heart rate, and arterial blood pressure of the rats between groups A, B and C were not significant （P^0.05）. No cerebral infarction was noted in group A, after reperfusion for 8 h, the volume of cere- bral infarction in group C was significantly smaller than that in group B （F = 15.32, P ~0.05）, but 24 h after reperfusion, the per- centage of volume of cerebral infarction between groups B and C was no significant dif-ference （P^0.05）. After reperfusion for 8 h, the number of positive ROS cells in group C was obviously less than ＂that in group B （F=25.72,P^0.05）, but no significant difference in after 24 h of reperfusion （P〉0.05）. Conclusion R＋PPX pretreatment can significantly reduce the generation of reactive oxygen species in the body and volume of cerebral infarction in the early stage, and thus play a protective effect on early brain injury caused by＇cerebral ischemia reperfusion.