Toll样受体4及干扰素-γ与乙肝病毒宫内感染相关性分析

Toll-like receptor 4 and IFN- γ intrauterine infection with the hepatitis B virus correlation analysis

目的探讨Toll样受体4及干扰素-γ与乙型肝炎病毒宫内感染之间的相关性。方法选取2013年1月至2015年6月在本院建册、产检、分娩且外周血乙肝标志物检测乙肝表面抗原(hepatitis B surface antigens,HBs Ag)、乙型肝炎E抗原(hepatitis B e antigens,HBe Ag)均为阳性的患者200例,其中发生胎儿宫内感染者100例,未发生胎儿宫内感染者100例,在分娩前测定外周血乙型肝炎病毒-DNA含量、外周血中干扰素-γ水平,分娩后留取脐带血测定HBs Ag和HBe Ag水平,通过免疫组化测定干扰素-γ和胎盘Toll样受体4表达情况,采用反转录聚合酶链反应测定胎盘中Toll样受体4的m RNA水平。结果分娩前感染组外周血乙型肝炎病毒的脱氧核糖核酸(HBV-DNA)水平高于未感染组,干扰素-γ水平低于未感染组;分娩后脐带血中感染组HBs Ag及HBe Ag水平均高于未感染组,脐带血中的干扰素-γ、Toll样受体4及其m RNA水平均低于未感染组。以上差异均具有显著性(P<0.05)。结论 Toll样受体4及血干扰素-γ在预防和减少乙型肝炎病毒透过胎盘屏障导致胎儿宫内感染中发挥重要作用。

Objective To investigate the correlation between Toll-like receptor 4 and IFN-γ intrauterine infection with the hepatitis B virus. Method January 2013 to June 2015 in our hospital for check-build delivery volumes peripheral markers of hepatitis B HBsAg,HBeAg were positive by 200 cases, 100 cases of intrauterine infection which occurs intrauterine infection has not occurred 100 patients, measured in the peripheral blood of hepatitis B virus before birth -DNA content in the peripheral blood levels of IFN-γ, measured specimens from cord blood after delivery HBsAg and HBeAg levels were measured IFN-γ and placenta samples by immunohistochemistry Toll 4 receptor expression using RT-PCR assay placenta Toll-like receptor 4 mRNA level.Result Childbirth infection in peripheral blood HBV-DNA levels higher than uninfected group （P〈0.05）, interferon-γ lower than uninfected group （P〈0.05）, umbilical cord after childbirth infection HBsAg and HBeAg levels were significantly higher than the uninfected group （P〈0.05）; after the birth of their umbilical cord infection interferon-γ, Toll-like receptor 4 and its mRNA levels were significantly lower than uninfected group （P〈0.05）.Conclusion The Toll-like receptor 4 and IFN -γ blood in the prevention and reduction of hepatitis B virus through the placental barrier leading to intrauterine infection play important roles.