Lasp1蛋白在乳腺浸润性癌组织中的表达及临床预后

Expression of Lasp1 protein in invasive breast cancer tissue and its significance in clinical prognosis

目的：探讨Lasp1蛋白在乳腺浸润性癌、乳腺导管内癌和正常组织中的表达及其与临床病理因素的关系,并进一步分析其表达对临床预后的影响。方法：应用免疫组织化学（En VisionTM）法分别检测Lasp1蛋白在70例乳腺浸润性癌组织、70例乳腺导管内癌组织和70例正常组织中的表达情况,对计数数据行卡方检验及Kaplan-meier法分析。结果：Lasp1蛋白在乳腺浸润性癌组织、乳腺导管内癌组织及正常组织中的阳性表达率分别为58.57%、28.57%、17.14%,3组之间表达差异有统计学意义（χ^2=28.26,P〈0.01）。乳腺浸润性癌组织中Lasp1蛋白的表达与肿瘤直径、淋巴结转移及雌激素（ER）有关（P〈0.05）,但与年龄、临床分期、孕激素（PR）及表皮生长因子受体2（HER-2）无关（P〉0.05）。Lasp1阳性表达的患者5年生存率低于Lasp1阴性表达的患者（χ^2=4.16,P〈0.05）。结论：乳腺浸润性癌组织中Lasp1蛋白的过表达不仅有利于判断癌细胞的浸润转移,而且对预测患者5年生存率也有参考价值。

Objective： To investigate the expression of Laspl protein in invasive breast cancer, breast intraductal carcinoma and normal tissue and its relationships with clinicopathologic parameters, and to further analyze its influence on clinical prognosis. Methods： The expression of Laspl protein was detected by immunohistochemisty （ EnvisionTM ） in invasive breast cancer tissues of 70 cases, 70 cases of breast intraductal carcinoma and in normal tissues of 70 cases. The enumeration data were tested byx2 test and the five-year survival prognosis was analysed by Kaplan-Meier test. Results： The positive expression rate of Laspl protein in invasive breast cancer tissue, breast intraductal carcinoma and normal tissue was respectively 58.57%, 28.57% and 17.14%, which showed statistically significant difference （χ2 = 28.26, P 〈 0. 01 ）. The positive expression of Laspl protein in invasive breast cancer tissue was related to tumor diameter, lymphnode metastasis and ER expression （P 〈 0. 05 ） , but not to age, clinical staging, PR expression and HER- 2 expression （ P 〉 0. 05 ）. The five- year survival rate of invasive breast cancer patients with positive expression of Laspl was lower than that of the patients with the negative expression with statistically significance difference （χ2 = 4. 16, P 〈 0. 05 ）. Conclusion： The over expression of Laspl in invasive breast cancer tissue is an important indicator both in the diagnosis of cancer cell infiltration and metastasis and in the prediction of five-year survival rate.