乳腺癌患者中HPV和MMTV的基因检测

Detection of HPV and MMTV in breast cancer cases

目的 在乳腺癌中检测人乳头瘤病毒和小鼠乳腺肿瘤病毒的存在,期望能为乳腺癌的基因治疗提供可能的靶点.方法 取76例乳腺癌石蜡组织,应用PCR和巢式PCR方法检测组织中HPV L1、HPV16E6、HPV16E7、HPV18E6和HPV18E7,MMTV的env基因序列,并进行了测序分析.结果 在76份样本中,HPV L1阳性7例（9.21％）,且测序后均为HPV18基因型;HPV16 E6和HPV16E7无阳性;HPV18 E6阳性5例（6.58％）;HPV18 E7阳性7例（9.21％）;MMTV的env基因阳性23例（30.26％）.结论 HPV18型相关基因与MMTV的env基因在乳腺癌中有存在,结果提示乳腺癌与HPV的相关性不是很高,但乳腺癌和MMTV或许有一定的相关性,也为乳腺癌的基因治疗提供了可能的靶点.

Objective To analyse the presence of human papillomavirus and mouse mammary tumor virus in breast cancer,and expected to provide a possible target for gene therapy of breast cancer.Methods PCR and nested PCR assay were used to detect the HPV L1,HPV16E6,HPV16E7,HPV18E6,HPV18E7 and the enevelop gene of MMTV,while sequence analysis was performed in 76 paraffin embedded tissues of breast cancer.Results The percentage of HPV L1,HPV18 E6,HPV18 E7 and the enevelop gene of MMTV was repectively 9.21%,6.58%,9.21% and 30.26%,while no HPV16 E6 and HPV16E7 was found in 76 paraffin embedded tissues of breast cancer.Conclusions HPV18 and the enevelop gene of MMTV was existed in breast cancer,there was low connection between HPV and breast cancer,but there may be some correlation between MMTV and breast cancer.This research offers a possible target for gene therapy of breast cancer.