养血清脑颗粒对血管性痴呆大鼠海马CA1区CD11b表达的影响

Effect of Yangxue Qingnao Granule on the Expression of CD11b in CA1 Region of Hippocampus of Vas-cular Dementia Rats

目的观察养血清脑颗粒对血管性痴呆(vascular dementia,VD)大鼠海马CA1区CD11b表达的影响,探讨养血清脑颗粒对小胶质细胞的调控作用。方法将144只SD大鼠随机分为假手术组、血管性痴呆模型组(模型组)及养血清脑颗粒治疗组(治疗组),采用改良的Pulsinelli四血管阻断法制备血管性痴呆大鼠模型,假手术组及模型组给予生理盐水[10 mL/(kg·d)]灌胃,治疗组以浓度为0.32 g/mL养血清脑颗粒10 mL/(kg·d)灌胃,每日1次,连续给药8周。分别在给药1、2、4、8周采用免疫组化法及Western blot法检测海马CA1区CD11b的表达水平。结果与假手术组比较,模型组各时间点大鼠海马CA1区CD11b蛋白表达均增高(P<0.01);与模型组比较,治疗组各时间点CD11b蛋白表达均下降(P<0.01),尤以2周时最明显。结论 VD大鼠海马CA1区小胶质细胞明显增生和活化,养血清脑颗粒可抑制小胶质细胞的增生和活化。

Objective To observe the effect of Yangxue Qingnao Granule（YQG） on the expression of CD11 b in CA1 region of hippocampus of vascular dementia rats, and to explore its regulation on microglias. Meth-ods Total y 144 SD rats were randomly divided into the sham-operation group, the vascular dementia model group（model）, and the YQG treated group（treated）. The vascular dementia rat model was prepared by modified Pulsinelli′s four-vessel occlusion. Rats in the sham-operation group and the model group were administered with normal saline（at the daily dose of 10 m L/kg） by gastrogavage, while those in the treated group were administered with YQG（0. 32 g/m L, at the daily dose of 10 m L/kg） by gastrogavage. Al administration was performed once per day for 8successive weeks. The expression of CD11 b in CA1 region of hippocampus of vascular dementia rats was detected at week 1, 2, 4, and 8, respectively. Results Compared with the sham-operation group, the expression of CD11 b in CA1 region of hippocampus of vascular dementia rats were significantly enhanced in the model group at each time point（P〈0. 01）. Compared with the model group, the expression of CD11 b in CA1 region of hippocampus of vascular dementia rats significantly decreased in the treated group at each time point（P〈0. 01）, especially at week 2.Conclusion Obvious activation and proliferation of microglias could be seen in CA1 region of hippocampus of vascular dementia rats, and YQG could inhibit activation and proliferation of microglias.