大野荞不同器官可溶性糖的抗氧化能力

Antioxidant Capacity of the Total Soluble Sugar from Various Organs of Fagopyrum megaspartanium

用蒽酮比色法测定大野荞(Fagopyrum Megaspartanium)不同器官中可溶性糖的含量,并采用NBT光化还原法、Fenton反应法和钼酸铵显色分光光度法研究了大野荞不同器官(茎、叶、花和种子)可溶性糖体外抗羟基自由基(·OH)、超氧阴离子自由基(·O_2^-)和过氧化氢(H_2O_2)的能力。结果表明:大野荞不同器官可溶性糖体外均能抗·OH、·O_2^-和H_2O_2。抗·OH的能力为:花、种子>茎、叶;抗·O_2^-的能力为:花>叶、种子、茎;抗H_2O_2的能力为:花、叶>茎>种子。

Total soluble sugar in different organs of Fagopyrum megaspartanium was extracted and determined by anthrone colorimetric method. And the capacity of scavenging·OH,·O2^- and H2O2 of total soluble sugar extracts from four organs（ aerial stem, leaf, flower and seed） of Fagopyrum megaspartanium was also studied by means of nitro blue terra-zolium photochemica reduction, Fenton reaction and spectrophotometric assay. The results showed that total soluble sugar extracts had the capacity of resisting·OH,·O2^- and H2O2. The capacity of resisting ·OH was sorted as： flower, seedaerial stem, leaf. The capacity of resisting·O2^- was sorted as： flowerleaf, seed, aerial stem. The capacity of resisting H2O2 was sorted as： flower, leafaerial stemseed.