摘要
以自提的玉米须多糖(CSPL)为原料制备玉米须硒多糖(Se-CSPL),研究Se-CSPL抗氧化活性并与CSPL进行比较。采用Sephdex G-75对合成的Se-CSPL进行纯化和小鼠急毒试验后,用HPLC法测定对DPPH自由基的清除能力,分别用邻苯三酚法及Fenton体系测定各物质对O_2^-·和·OH的抑制作用。试验结果表明:Se-CSPL对·OH的清除能力稍强于CSPL,对O_2^-·的清除能力明显强于CSPL,当Se-CSPL的浓度大于1.0 mg/mL时,对DPPH自由基的清除率为94.9%与VC96.2%相近。说明Se-CSPL有良好的抗氧化活性,可以作为优良的抗氧化剂使用。
Selenium polysaccarides（ Se-CSPL） was synthesized with corn silk polysaccharide and Na2SeO3,then researched antioxidant activity of Se-CSPL were compared with CSPL. After Se-CSPL purified with Sephdex G-75 and mouse acute toxicity test, scavenging DPPH· activity was measured by HPLC. Determined inhibition on O2^-· and ·OH by pyrogallol method and Fenton system separately. The results showed that the scavenging·OH of Se-CSPL was little stronger than CSPL and scavenging O2^-·capacity was stronger than CSPL.When the concentration of Se-CSPL greater than 1.0 mg/m L, scavenging DPPH·radical rate of 94.9 % was similar to VC of 96.2 %. Se-CSPL has a good antioxidant activity, may be used as an excellent antioxidant.
出处
《食品研究与开发》
CAS
北大核心
2016年第7期37-40,共4页
Food Research and Development
基金
省卫生厅项目,玉米须多糖的硒化及其抗氧化降血糖抗肿瘤生物活性研究(2012-258)
关键词
玉米须
多糖
硒化反应
抗氧化
清除自由基
corn silk
polysaccaride
selenium chemical reaction
antioxidant activity
scavenging free radical
