MICA基因第2、3和4外显子多态性与血清阴性脊柱关节病关联研究

Association of MICA gene exon 2～4 polymorphism with seronegative spondylarthropathies

目的 探讨MICA基因外显子 2、3和 4的多态性与血清阴性脊柱关节病 (SpA)的发生是否存在关联。方法 采用地高辛标记的寡核苷酸探针杂交技术 (PCR SSOP) ,调查上海地区 199例B2 7(+)SpA患者MICA外显子 2、3和 4的多态性。结果 SpA病例组中MICA 0 0 7的频率比随机对照组显著升高 (RR =3 0 4 ,Pc=0 0 0 0 0 4 5 ) ;但比较B2 7(+)病例组与B2 7(+)正常对照组差异无显著性 (P >0 0 5 )。结论 MICA 0 0 7等位基因与SpA的发生可能无直接的关联 ,MICA 0 0 7的频率在SpA病例组中显著升高可能由于和HLA B2 7基因存在连锁不平衡所致。

Objective To investigate whether MICA gene exon 2,3 and 4 polymorphism is associated with seronegative spondylarthropathies (SpA) or not in Chinese Han population.Methods All 199 B27 positive patients with SpA and 183 randomly ethnically matched healthy controls,and 12 B27 positive controls were enrolled to detect the MICA genotype from its exons 2,3 and 4 by using PCR SSOP method in Shanghai area.Results The MICA007 allele frequency was significantly more in the patient group (18 0%) than in the randomly healthy control group (6 6%) (RR=3 04, P =0 000 045).However,the MICA007 allele frequency was not significantly higher in the B27 positive patient group than in the B27 positive control group.Conclusion The MICA007 allele itself may not be the real disease susceptibility gene involved in the development of ankylosing spondylitis.The increased frequency of MICA007 allele is supposed to be due to a strong linkage disequilibrium between MICA and HLA B genes.