氟剂对成骨细胞成骨功能表达的影响

Effect of fluoride on the function of osteoblast bone formation

目的 从不同剂量氟化钠 (NaF)对大鼠成骨细胞 (OB)功能表达的影响 ,研究氟剂促进成骨作用的机制及剂量效应关系。方法 经酶消化法从新生 2 4hSpregne Dawley (SD)种乳鼠头盖骨分离得到的成骨细胞。在 10 -7～ 5× 10 -4mol/LNaF中培养。用生化法测定细胞碱性磷酸酶 (ALP)活性 ,用放射免疫法测定培养液中骨钙素 (OC)含量。结果 NaF对细胞ALP活性的影响呈双向 ,即低浓度NaF(10 -7～ 10 -5mol/L)增加ALP活性 ,高浓度NaF (10 -4～ 5× 10 -4mol/L)则抑制ALP活性 ;对骨钙素产生和分泌的影响则呈单向 ,即各浓度NaF均刺激细胞骨钙素分泌 ,并与氟剂剂量呈正相关 ,其中 5× 10 -4mol/L组骨钙素水平最高 ,与对照组相比增加 6 7 14 % (P <0 0 5 )。结论 适量氟剂能增加成骨细胞ALP的活性及骨钙素的产生 ,促进成骨细胞的骨形成功能 。

Objective To observe the effects of different concentration of NaF on osteoblasts function of bone formation and dose effect relationship.Methods Osteoblasts were obtained from the calvaria of 24 hour newborn SD rats by enzymic degestion and cultuered in the medium with 10 -7 ～5× 10 -4 mol/L NaF.The ALP activity and osterocalcin were examined by biochemical assay and radioimmunologic assay (RIA) respectively.Results Results showed that the effect of NaF on ALP activity was in a double phase manner,e.g. low concentration NaF(10 -7 ,10 -6 ,10 -5 mol/L) increased ALP activity ( P <0 05) respectively,vice versa the high concentration (10 -4 ,5×10 -4 mol/L) decreased ALP activity ( P <0 05).However,the action of NaF on osterocalcin secretion appeared different from ALP activity.All above concentrations of NaF showed the stimulated effect on the secretion of osterocalcin.The highest secret rate was up to 67 14% at 5×10 -4 mol/L NaF.( P <0 05).Conclusion Quantum satis fluoride can increase osteoblasts ALP activity and osteocalcin production, facilitate osteoblasts osteogenesis function,but the range of effective dose of fluoride is very narrow.