肿瘤坏死因子-β基因多态与系统性红斑狼疮关系研究

An association between gene polymorphism of tumor necrosis factor β and systemic lupus erythematosus

目的 探讨肿瘤坏死因子 (TNF) β的基因多态与华东地区汉族人系统性红斑狼疮(SLE)易感性的关系及其在SLE家系中的传递规律。方法 运用聚合酶链式反应 限制性片段长度多态 (PCR RFLP)对 99例SLE患者、116名正常人及 12个SLE家系的 4 8名成员进行了TNF β基因分型。结果 SLE患者的TNF β 2等位基因频率高于对照组 (6 2 6 % / 4 7 0 % ,OR =1 89,P =0 0 0 12 ) ,但可能样本量过小 ,在SLE家系研究中未发现TNF β基因多态存在关联的证据以及传递 /连锁不平衡的证据。结论 TNF β

Objective To make sure whether there is any association between genetic polymorphism of tumor necrosis factor (TNF) β and systemic lupus erythematosus (SLE).Method PCR RFLP was used.A population based and family based study was carried out in 99 SLE patients,116 health controls and 12 families.Results The TNF β2 allele frequency of SLE patients was significantly different from that of controls ( P <0 05).The subjects with TNF β2 allele tend to be more susceptible to SLE than those without.Conclusion The results of family based association analysis,TDT and S TDT suggest that no linkage is found between TNF β and SLE.The subjects with TNF β2 allele tend to be more susceptible to SLE than those without.