依达拉奉对老年大鼠术后早期记忆水平的影响及其机制研究

Effects of edaravone on early memory of rats with its mechanism research

目的:探索使用依达拉奉对老年大鼠术后早期记忆水平的影响。方法:将老年大鼠随机分为空白对照组、手术组和依达拉奉组,依达拉奉组与手术组采用大鼠剖腹探查模型,依达拉奉组于手术结束时肌注依达拉奉3 mg·kg-1,对照组不做手术处理。各组大鼠于手术后12、24、72 h取静脉血浆,立即按照S100β试剂盒、神经元特异性烯醇化酶(neuron-specific enolase,NSE)试剂盒说明书测神经损伤特异性标志物S100β蛋白和NSE含量。采用条件恐怖适应实验中追踪适应实验检测各组大鼠术后记忆能力。各组大鼠于术后72 h最后一次采血后断头取脑,分离海马,采用ELISA检测大鼠海马IL-1β、TNF-α浓度。结果:与对照组相比,手术可导致老年大鼠术后血浆S100β蛋白、NSE表达上升(P<0.01),海马IL-1β、TNF-α表达增多(P<0.01),记忆水平下降(P<0.01)。使用依达拉奉可抑制手术引起的老年大鼠血浆S100β蛋白、NSE表达上升(P<0.01)和海马IL-1β、TNF-α表达增多(P<0.01),减轻手术引起的老年大鼠记忆能力下降(P<0.01)。结论:手术可导致老年大鼠海马炎症反应及神经细胞损伤,引发老年大鼠早期记忆水平下降,使用依达拉奉可减轻手术引起的老年大鼠海马炎症反应及神经细胞损伤,减轻手术引起的老年大鼠早期记忆水平下降。

Objective：To investigate the effects of edaravone on early memory of rats and its mechanonism. Methods：Aged rats were randomly allocated to the following contral（C）group, abdominal surgery alone（S）group, or intramuscular injection of edaravone after surgery（E）group. Rats received abdominal surgery in S group and E group. Rats in E group received intraperitoneally injection of edaravone （3mg·kg^-1） after abdominal surgery. The concentration of S100β and NSE in plasma were measured by ELISA 12,24,72h after operation. Hippocampal IL-1β,TNF-α were measured by ELISA 72h after operation.Rats＇ memory was assessed by Fear Conditioning Test 24 h after surgery. Results：Compared with that of control group, the concentration of S100β and NSE in plasma enhanced after surgery （P〈0.01）but diminished if using edaravone（3mg·kg^-1） （P〈0.01）.Compared with that of control group, the expression of IL-1β,TNF-α in rats＇ hippocampa enhanced after surgery （P〈0.01）but diminished if using edaravone （3mg·kg^-1） （P〈0.01）. Surgery could result in rats＇ memory impairment（P〈0.01）but diminished if using edaravone （3mg·kg^-1） （P〈0.01）. Conclusion：Edaravone can restrain central inflamaton and injury of nerve to diminish the memory impairment after surgery in rats.