阿托伐他汀对动脉粥样硬化大鼠热休克蛋白60、核转录因子-κB表达的影响

Effect of atorvastatin on heat shock protein 60 and NF-κB expression in SD rats with atherosclerosis

目的:探讨阿托伐他汀对动脉粥样硬化大鼠主动脉及心肌热休克蛋白60(HSP60)及核转录因子-κB(NF-κB)表达的影响。方法:选取普通级SD雄性大鼠18只,分为健康对照组(6只)、模型组(6只)和阿托伐他汀干预组(6只)。对照组给予基础饲料喂养;模型组给予高脂饲料喂养;阿托伐他汀干预组给予高脂饲料喂养12周后,阿托伐他汀干预4周(10 mg·kg^(-1)·d^(-1)灌胃)。实验终点处死大鼠,腹主动脉取血检测各组TC、TG、LDL-C,HE染色观察各组主动脉的病理改变,免疫组织化学法检测主动脉及心肌HSP60、NF-κB的蛋白表达。结果:模型组大鼠TC、LDL-C均高于对照组(均P<0.01)。模型组大鼠主动脉组织内可见动脉粥样斑块形成;主动脉及心肌HSP60、NF-κB的表达均高于对照组(均P<0.01)。阿托伐他汀干预组大鼠TC、LDL-C均低于模型组(均P<0.01);主动脉组织内动脉粥样硬化斑块病变减轻;主动脉及心肌HSP60、NF-κB的表达均低于模型组(均P<0.05)。结论:阿托伐他汀可调节血脂,降低HSP60的表达;并通过抑制NF-κB信号通路,减轻动脉粥样硬化病变。

Objective：To explore the effect of atorvastatin on heat shock protein 60（HSP60）and NF-κB signal pathway expression in aorta and heart tissue of atherosclerosis rats.Method：Eighteen SD rats were randomly divided into three groups：control group（n=6）fed with normal diet;atherosclerosis group（n=6）fed with high fat diet;atorvastatin group（n=6）fed with high fat diet for twelve weeks and then giving atorvastatin（10mg· kg^-1·d^-1 per gavage）for four weeks.Then blood was collected for lipid measuring,aorta tissue was prepared for morphologic study（HE）,aorta and heart tissues were prepared for detecting the expression of HSP60 and NF-κB by immunohistochemical analysis.Result：Compared with control group,serum TC and LDL-C levels were significantly higher in atherosclerosis group（both P〈0.01）,atherosclerotic lesion was apparent,the expression of HSP60 and NF-κB significantly increased in aorta and heart tissues（all P〈0.01）.Compared with atherosclerosis group,serum TC and LDL-C levels in atorvastatin group were significantly lower（both P〈0.01）,and atherosclerotic lesion was lightened,HSP60 and NF-κB significantly decreased in aorta and heart tissues（all P〈0.05）.Conclusion：Atorvastatin could reduce blood lipid,decrease the expression of HSP60,and inhibit NF-κB signal pathway,which contributes to preventing and alleviating atherosclerosis.