microRNA-1与microRNA-126在支气管哮喘急性发作期患儿外周血中的表达及诊断价值

The Expression of microRNA-1 and microRNA-126 in Peripheral Blood of Asthmatic Children with Acute Episode of Bronchial Asthma and its Correlation with the Onset of the Disease

目的:探讨microRNA-1与microRNA-126在支气管哮喘急性发作期患儿外周血中的表达及其与患儿发病的相关性。方法:临床纳入2013年1月至2015年1月我院收治的支气管哮喘急性发作期患儿48例,设为哮喘组,同时纳入同时期内52例健康体检儿童作为对照组。分别采集两组外周血,并进行microRNA-1与microRNA-126水平检测,同时检测IL-4、INF-γ水平。结果:哮喘组患儿IL-4、INF-γ水平分别为(109.65±74.31)ng/L、(70.47±11.96)ng/L,对照纽患儿IL-4、INF-γ水平分别为(78.47±75.78)ng/L、(77.05±17.21)ng/L,差异有显著性(P<0.05);哮喘组患儿microRNA-1与microRNA-126水平分别为(2.15±0.97)、(7.34±1.26),对照组患儿microRNA-1与microRNA-126水平分别为(5.81±1.29)、(3.66±0.91),差异有显著性(P<0.05);microRNA-126、microRNA-1在支气管哮喘急性发作期患儿外周血中的灵敏度与在对照组的特异度分别为85.42%、79.17%、78.85%、73.08%;ROC曲线下面积分别为0.917、0.865。结论:支气管哮喘急性发作期患儿外周血中microRNA-126水平升高,microRNA-1水平降低,两者可以作为临床诊断儿童支气管哮喘急性发作的客观指标。

Objective：To investigate the expression of microRNA-1 and microRNA-126 in peripheral blood in children with acute episode of bronchial asthma and its correlation with the onset of the disease.Methods：48 patients with acute exacerbation of bronchial asthma in our hospital from Jan.2013 to Jan.2015 were included in the asthma group,while the control group was included 52 cases of healthy children.The peripheral blood of the two groups were collected,and the level of microRNA-126 and microRNA-1 was detected,and the levels of IL-4 and INF—y were detected as the same time.Results：The levels of IL- 4,INF-γ in asthma group were（109.65±74.31） ng/L and（70.47±11.96）ng/L,while those in control group were（78.47±75.78）ng/L and（77.05±17.21）ng/L,the difference was significant（P〈 0.05）.The levels of microRNA-1,microRNA-126 in asthma group were（2.15±0.97） and（7.34±1.26）,the levels of microRNA-1,microRNA-126 in control group were（5.81±1.29） and（3.66±0.91）,the difference was significant（P〈 0.05）.The sensitivity and specificity of microRNA-126 and microRNA-1 in peripheral blood of children with acute attack of bronchial asthma were 85.42%,78.85%,79.17%and 73.08%respectively,and the area under the ROC curve was 0.917 and 0.865 respectively.Conclusion;The level of microRNA-126 in peripheral blood of asthmatic children with acute attack of bronchial asthma is increased,and the level of microRNA-1 is decreased,and both can be used as an objective indicator of acute attack of bronchial asthma in children.