人甲型H7N9禽流感全病毒灭活疫苗在小鼠中的免疫原性

Immunogenicity of inactivated human avian influenza A H7N9 virus vaccine in mice

目的评价人甲型H7N9禽流感全病毒灭活疫苗在小鼠中的免疫原性。方法分别将H7N9与H1N1、H5N1全病毒灭活疫苗稀释至血凝素抗原(hemagglutinin,HA)浓度分别为30、6、1.2、0.24和0.048μg/ml后,免疫BALB/c小鼠,各10只,0.5 ml/只,采用血凝抑制(hemagglutinin inhibition,HI)试验检测免疫后小鼠血清抗体滴度,计算抗体几何平均滴度(GMT)及半数有效剂量(ED_(50)),并检测免疫后小鼠血清对其他亚型病毒的HI滴度。结果 H7N9组小鼠阳转率与H1N1组较相近,仅H7N9 0.6μg剂量组GMT明显低于H1N1同剂量组(P<0.001),其余剂量组差异无统计学意义(P>0.05);H7N9与H1N1、H5N1全病毒灭活疫苗免疫小鼠ED_(50)分别为0.27、0.12和15μg HA。免疫后血清对其他亚型病毒无交叉反应。结论 H7N9疫苗在小鼠中的免疫原性略低于H1N1疫苗,但明显优于H5N1疫苗。

Objective To evaluate the immunogenicity of inactivated human avian influenza A H7N9 vaccine in mice.Methods Inactivated whole influenza virus H7N9,H1N1 and H5N1 vaccines were diluted to hemagglutinin（HA）concentrations of 30,6,1.2,0.24 and 0.048 μg / ml separately,with which BALB / c mice were immunized,0.5 ml for each.The serum antibody titers of mice were determined by hemagglutinin inhibition（HI）test,based on which GMT and median effective dose（ED50）was calculated,and the HI titer of murine sera against influenza virus of other subtypes were determined.Results The positive conversion rate of antibody of mice in H7N9 group was similar to that in H1N1 group.The GMTs of mice immunized with 0.6 μg H7N9 virus were significantly lower than（P〈.001）,while those at other dosages showed no significant difference with（P〈.05）,those of mice immunized with H1N1 virus at the same dosages.The ED50 of inactivated H7N9,H1N1 and H5N1 virus vaccines in mice were 0.27,0.12 and 15 μg HA respectively.The immunosera showed no cross-reaction with virus of other subtypes.Conclusion The immunogenicity of inactivated H7N9 vaccine in mice was slightly lower than that of H1N1 vaccine,while was significantly higher than that of H5N1 vaccine.