氯霉素在p-PTA／CS-AB／GCE修饰电极上的电化学行为及测定

Determination of Chloramphenicol at Poly Phospho-tungstic Acid/Chitosan-Acetylene Black Modified Electrode and Its Electrochemical Behaviors

制备了聚磷钨酸／壳聚糖-乙炔黑修饰电极（p-PTA／CS—AB／GCE），采用循环伏安法（CV）研究了氯霉素在该修饰电极上的电化学行为。结果表明，在pH6．0的PBS溶液中，氯霉素（CAP）在该修饰电极上出现1个还原峰，在40—400mV／s扫速范围内，CAP的还原峰电流与扫速呈线性关系，说明CAP在修饰电极上的电化学反应过程是受吸附控制的不可逆过程。用差分脉冲伏安法（DPV）对不同浓度的CAP进行检测，在5．0×10^-71．0×10^-4mol／L浓度范围内，还原峰电流与浓度呈线性关系，检出限（S／N=3）为5．13×10^-8mol／L。用该方法对氯霉素片进行检测，相对标准偏差（RSD）为1．4％，回收率为97．7％～105．1％。

A new modified electrode （p-FFA/CS- AB/GCE ） was prepared with phospho-tungstic acid, chitosan and acetylene black. The electrochemical behaviors of chloramphenicol （CAP） on p- PTA/CS -AB/GCE were investigated by cyclic vohammetry（CV）. The results showed that there was an obvious reduction peak of CAP on the modified electrode in pH 6.0 PBS buffer solution and the re- duction peak current was linear to the scan rate in the range of 40 -400 mV/s, indicating that the e- lectrode process of CAP was controlled by absorption. The different concentrations of CAP were deter- mined by differential pulse voltammetry, and a good linear relationship was observed between reduction peak current and CAP concentration in the range of 5. 0 × 10^-7 - 1.0 × 10^-4 mol/L. The detection limit （S/N =3） was 5. 13 × 10^-8 mol/L. The mothed was applied in the determination of CAP in chloramphen- icol tablet sample, with relative standard deviation of 1.4% and recoveries of 97. 7% - 105. 1%.