固相萃取/超高效液相色谱法测定纺织品中14种紫外吸收剂

Determination of 14 Kinds of Ultraviolet Absorbers in Textiles by Solid Phase Extraction Combined with Ultra-high Performance Liquid Chromatography

建立了固相萃取（SPE）净化，超高效液相色谱（UPLC）同时测定纺织品中14种二苯甲酮类（BPs）和苯并三唑类（BZTs）紫外吸收剂的分析方法。样品以甲醇-四氢呋喃超声提取，提取液经ENVI—Carb固相萃取柱净化后，在C，。色谱柱和乙腈-0．1％甲酸水流动相下梯度洗脱分离，二极管阵列检测器测定。结果表明，14种紫外吸收剂在0．1～50mg／L浓度范围内线性关系良好，相关系数均大于0．999；方法检出限为1—5mg／kg；阴性涤纶和腈纶样品在3个加标水平下的平均回收率（n=6）为81．5％～99．1％，相对标准偏差（RSD）为2．8％-7．5％。该方法简单快速，回收率和精密度良好，适用于纺织品中14种紫外吸收剂的同时测定。

A new method was established for the simultaneous determination of fourteen benzophenone and benzotriazole UV absorbents in textiles by solid phase extraction（SPE） combined with ultra-high performance liquid chromatography（UPLC）. The samples were extracted with methanol- tetrahydro- furan by ultrasonic. After purified using ENVI-Carb SPE column, the extract solutions were separa- ted on a C18 chromatographic column using acetonitrile -0. 1% formic acid as mobile system by gradi- ent elution, and detected with diode array detector （DAD）. The results indicated that 14 analytes presented favorable linearity（r 〉0. 999） in the concentration range of 0. 1 -50 mg/L. The limits of detection of the method （ LODs, S/N = 3 ） were in the range of 1 - 5 mg/kg. Recoveries were calcu- lated to be in the range of 81.5% -99. 1% at three spiked concentration levels in negative samples, with relative standard deviations （ RSD, n = 6 ） of 2. 8% - 7.5%. The method is simple, rapid, and is suitable for the simultaneous determination of fourteen UV absorbents in textiles with favorable recoveries and precision.