超高效液相色谱法测定涂料中11种紫外吸收剂

Determination of 11 Kinds of Ultraviolet Absorbers in Coatings by Variable Flow Elution Combing with Ultra High Performance Liquid Chromatography

建立了同时测定涂料中11种苯甲酮类和苯并三唑类紫外吸收剂的超高效液相色谱（UPLC）分析方法。乳胶漆类样品经水分散后，乙酸乙酯提取；木器清漆类样品以乙腈直接提取。提取液以C。。色谱柱为分离柱，在乙腈-水流动相体系下变流速洗脱分离，二极管阵列检测器检测。结果表明，11种紫外吸收剂在0．1～50mg／L浓度范围内线性关系良好，相关系数（r）均不小于0．9990；方法检出限为10～30mg／kg；3个加标水平下的平均回收率（n=6）为80．7％-107％，相对标准偏差（RSD）为3．0％～8．7％。该方法简单、快速，回收率和精密度良好，适用于涂料中11种紫外吸收剂的同时测定。

An ultra high performance liquid chromatographic（UPLC） method was established for the simultaneous determination of eleven benzophenone and benzotriazole UV absorbents in coating sam- ples. The emulsion paint samples were firstly dispersed with water, and subsequently extracted with ethyl acetate, while the wood varnish samples were extracted directly with acetonitrile. The extracts were separated on a Cls chromatographic column using acetonitrile -water mobile system with a varia- ble flow rate, and detected with diode array detector（DAD）. The results indicated that 11 analytes had good linear relationships（r≥0. 999 0） in the concentration range of 0. 1 -50 mg/L. The limits of detection of the method（MLODs） were in the range of 10 -30 mg/kg. Recoveries of 11 analytes at three spiked concentration slevels were in the range of 80. 7% - 107% with relative standard deviations （RSD, n = 6） of 3. 0% - 8. 7%. The method was simple and rapid, and was suitable for the simultane- ous determination of eleven UV absorbents in coating samples with favorable recoveries and precisions.