新生猪溶血性黄疸模型的制备与验证

Establishment and validation of a neonatal pig model of hemolytic jaundice

目的建立新生猪溶血性黄疸动物模型,以进一步研究新生儿溶血性黄疸的病理生理。方法 7日龄纯种大约克白猪分成实验组和对照组,每组各6只。并采用免疫新西兰大白兔的方法制备兔抗猪红细胞抗体,分离兔抗猪红细胞血清。实验组静脉注射兔抗猪红细胞血清5 m L,对照组注射5 m L生理盐水。两组均每6 h采血送检血常规及肝功能。结果实验组注射兔抗猪红细胞血清18 h后血清胆红素水平高于对照组(64±30μmol/L vs 20±4μmol/L,P<0.05),48 h达高峰(275±31μmol/L),96 h明显降低(95±17μmol/L),均高于对照组(P<0.05)。注射抗血清后18 h,实验组红细胞降至(4.58±0.32)×10^(12)/L,低于对照组[(5.09±0.44)×10^(12)/L](P<0.05);24 h实验组红细胞[(4.21±0.24)×10^(12)/L]和HB(87±3 g)进一步降低,低于对照组[(5.11±0.39)×10^(12)/L,97±6 g](P<0.05);二组间的红细胞、血红蛋白差异在36~48 h达高峰。结论新生猪溶血性黄疸模型较好地模拟了人类溶血性黄疸的病理过程,为更进一步研究新生儿溶血病提供了良好的生物物质基础。

Objective To establish a neonatal pig model of hemolytic jaundice.Methods Twelve sevenday-old purebred Yorkshire pigs were randomly divided into an experimental group and a control group（n=6 each）.Immunization of New Zealand white rabbits was used to prepare rabbit anti-porcine red blood cell antibodies,and rabbit anti-porcine red blood cell serum was separated.The neonatal pigs in the experimental group were given an intravenous injection of rabbit anti-porcine red blood cell serum（5 m L）,and those in the control group were given an intravenous injection of normal saline（5 m L）.Venous blood samples were collected every 6 hours for routine blood test and liver function evaluation.Results The experimental group had a significantly higher serum bilirubin level than the control group at 18 hours after the injection of rabbit anti-porcine red blood cell serum（64±30 μmol/L vs 20±4 μmol/L;P〈0.05）.In the experimental group,the serum bilirubin level reached the peak at 48 hours（275±31 μmol/L）,and decreased significantly at 96 hours after the injection（95±17 μmol/L）,but all significantly higher than that in the control group（P〈0.05）.At 18 hours after the injection,the experimental group had a significantly lower red blood cell（RBC）count than the control group [（4.58±0.32）×10^12）/L vs（5.09±0.44）×10^12）/L;P〈0.05];at 24 hours,the experimental group showed further reductions in RBC count and hemoglobin level and had significantly lower RBC count and hemoglobin level than the control group [RBC：（4.21±0.24）×10^12）/L vs（5.11±0.39）×10^12）/L,P0.05;hemoglobin：87±3 g vs 97±6 g,P〈0.05].The differences in RBC count and hemoglobin level between the two groups were largest at 36-48 hours.Conclusions The neonatal pig model of hemolytic jaundice simulates the pathological process of human hemolytic jaundice well and provides good biological and material bases for further investigation of neonatal hemolysis.