小五台山蟹蛛DNA条形码分子鉴定

Molecular identification of crab spiders from Xiaowutai Mountains,China with DNA barcoding

为了探讨DNA条形码技术在蟹蛛科蜘蛛物种鉴定中的可行性,本研究基于线粒体COI基因序列,使用邻接法和贝叶斯法构建系统发育树,ABGD(automatic barcode gap discovery)软件对样本进行划分,对小五台山25种蟹蛛110个样本进行DNA条形码分子鉴定.结果表明:邻接法和贝叶斯法构建的系统发育树聚类结果与ABGD软件划分结果以及形态分类鉴定结果相一致.据此笔者认为DNA条形码作为一种有效的分子鉴定工具可以应用到蟹蛛科蜘蛛物种鉴定中.

In order to explore the feasibility of DNA barcoding in the species identification of crab spiders,the COI gene of 110 thomisid samples belonged to 25 species in Xiaowutai Mountains,China,was applied;the phylogenetic trees were reconstructed by neighbor-joining（NJ）and Bayesian Inference（BI）methods,and with ABGD（automatic barcode gap discovery）software to divide the samples.The results showed that the samples belonging to the same species clustered in one monophyly and all species were successfully distinguished by the phylogenetic trees,and the groups automatically defined by ABGD perfectly matched the groups defined by morphological characters,and accorded with phylogenetic trees.We believe DNA barcoding can be used as an effective tool in the molecular identification of crab spiders.