IL-1β及IL-1RN intron2基因多态性在中国山东地区汉族人大肠癌中的分布特征

Distribution of IL-1β and IL-1RN intron2 gene polymorphism in colorectal cancer of Chinese Han people in Shandong area

目的探讨白细胞介素-1β（IL-1β）及白细胞介素-1RN（IL-1RN）基因多态性与中国山东地区汉族人大肠癌的关系。方法用基因芯片检测IL-1β-31／-511位点单核苷酸多态性（SNP）；以凝胶电泳检测IL-1RNintron2基因多态性（VNTR）；以ELISA测定IL-1β／-1ra血清水平。以病例一对照研究方法分析490例大肠癌与IL-1β-31／-511SNP及IL-1RNVNTR的关系。用矿、t检验行相关指标的比较。以比值比（OR）及其95％可信区间（95％CI）估计相对风险。结果（1）大肠癌组IL—1ra／-1β比值显著低于对照组（M=-23，P〈0．01）；IL-1β-5111TT及T频率均显著高于对照组[X^2=5．22，P〈0．05）比（X^2=4．59，P〈0．05）]。（2）直肠癌及管状腺癌组．511TT／T频率均显著高于对照组，其OR（95％CI）分别为，TF：1．59（1．03～2．45）及1．74（1．19～2．55）；T：1．39（1．04～1．86）及1．42（1．10-1．83）。低分化管状腺癌组-511TT频率（61．90％）显著高于对照组；而其cT频率（19．05％）显著低于对照组，其OR（95％CI）分别为，TT：4．57（1．96—10．65）及CT：0．23（0．08—0．64）；中及低分化管状腺癌组-511T频率均显著高于对照组，其OR（95％CI）分别为：5．80（1．24—27．08）及2．35（1．24—4．59）。结论IL．1B．511C／TSNP与山东地区汉族人大肠癌相关。

Objective To study the distribution characteristics of IL-1β-31/-511 single nucleotide polymorphisms（SNPs） and IL-1RN intron2 variable number of tandem repeat （VNTR） of Chinese Han people with colorectal cancer in Shandong area. Methods The IL-1β-31/-511SNP in a total of 490 colorectal cancers were determined by using gene chip. The VNTR were investigated by using PCR- RFLP. The sera concentrations of IL-1β/-1ra were measured by ELISA. The relationship between IL-1β-31/-511SNP or IL-1RN VNTR and colorectal cancer group in 490 cases were analyzed by case-control study.x2 or t test was used to compare the results of relevant index. The relative risk degree was estimated by odds ratio （OR） and 95% confidence interval （95% CI）. Results The ratio of IL-1 ra/IL-1β was significantly lower than that of the control group （ u = - 23,P 〈 0. 01）. Frequencies of IL- 113-511TT（P 〈0. 05 ,OR = 1.55,95% CI = 1.10 -2. 18） and T allele （P 〈0. 05 ,OR = 1.31,95% CI = 1.05 - 1.63 ） in patients with colorectal cancer were significantly higher than that in controls correspondingly. - 511TF and T frequency in rectal cancer or tubular adenocarcinoma was significantly higher than control group, The ORs （95% CI） appeared to be 1.59 （1.03 -2.45）,1.74 （1.19 -2.55） and 1.39（1.04 - 1.86）, 1.42 （1.10-1.83 ）respectively. In poorly differentiated tubular gland cancer group, -511TT frequency （61.90%）was higher than that of the control group. In contrast, the frequency of CT （ 19.05% ） was on the opposite tendency of TT. The ORs （95% CI） appeared to be 4. 57 （ 1.96 - 10.65） and 0. 23 （0. 08 -0. 64） respectively. -511T frequency of moderate or low differentiated group of tubular gland cancer were significantly higher than the control group, The ORs （95% CI） appeared to be 5.80 （ 1.24 - 27.08 ） and 235 （1.24 -4. 59）, respectively area. Conclusion IL-1β-511C/T SNP is associated with the occurrence and development of eoloreetal cancer of Chinese Han people in Shandong area.