云南省罗平县与腾冲县两地稻瘟病菌致病类型分析

Pathotype Analysis of Magnaporthe oryzae in Luoping and Tengchong of Yunnan Province

为了明确滇东南和滇西水稻稻瘟病菌组成,采用苗期喷雾法将来自2012年和2013年罗平县与腾冲县2个病圃的295个单孢菌株,接种到丽江新团黑谷、CO39和IRBL三套单基因系,测定它们的致病类型。结果表明,295个菌株可划分为79个致病类型,其中罗平县48个,特异类型32个;腾冲县47个,特异类型31个,两地共有类型仅16个,占5.42%;携带Pi-9(t)、Pi-2(t)、Pi-12(t)等3个抗病基因的水稻品种的被侵染率分别为10.13%、11.39%、11.39%,表明这些抗病基因在抗病育种工作中仍有很好的利用价值。

To clarify the components of Magnaporthe oryzae in southeastern and western Yunnan Province, 295 single spore isolates collected from the nurseries in Luoping and Tengchong counties in 2012 and 2013 wereinoculated to three sets of single-isogenic lines（SILs） including LTH, CO39 and IRBL, with seedling sprayingmethod. The pathotypes were determined. The results showed that 295 isolates could be classified into 79 pathotypes, among which, there were 48 pathotypes with 32 special types in Luoping and 47 pathotypes with 31 special types in Tengchong. There were only 16 pathotypes shared in the two nurseries, accounted for 5.42%.The infected frequencies of the cultivars with Pi-9（t）, Pi-2（t） or Pi-12（t） blast-resistance genes were 10.13%,11.39% and 11.39%, indicating that those genes would be useful in blast-resistant breeding.