基于血管内皮生长因子及其受体途径的局灶节段性肾小球硬化大鼠的艾灸干预随机对照实验

Randomized controlled trial of moxibustion intervention in FSGS rats based on the expression of vascular endothelial growth factor and its receptor

目的:观察在艾灸干预下局灶节段性肾小球硬化(FSGS)大鼠肾组织中血管内皮生长因子(VEGF)及其受体(VEGFR-2)的表达。方法:102只健康雄性SD大鼠,按体质量随机分为正常组7只、假手术组7只、造模组88只。采取间隔2周2次尾静脉注射阿霉素并单侧肾切除的方法建立FSGS大鼠模型。造模成功后大鼠根据尿蛋白定量随机分为模型组、氯沙坦组、肾俞灸法(短时、中时、长时)组和膈俞灸法(短时、中时、长时)组,每组11只,各组采用相应干预。12周后运用免疫组化法检测VEGF、VEGFR-2、ET-1、AngⅡ、AT1R在肾组织的蛋白表达;硝酸还原酶法检测NO在肾组织的含量。结果:与假手术组比较,模型组VEGF、VEGFR-2、ET-1、AngⅡ、AT1R蛋白表达显著升高,NO含量显著降低(P<0.01);与模型组比较,氯沙坦组、肾俞长时组和膈俞长时组VEGF、VEGFR-2、ET-1、AngⅡ、AT1R表达降低,NO含量升高(P<0.05,P<0.01)。结论:艾灸可能通过下调VEGF及其受体VEGFR-2的蛋白表达起到保护肾组织的作用。

Objective:To observe the effects of moxibustion intervention on treating focal segmental glomerulosclerosis(FSGS) rat model by observing the expression of vascular endothelial growth factor(VEGF) and its receptor(VEGFR-2) in renal tissue.Methods:A total of 102 SD rats were randomly divided into the normal group(n=7), sham operation group(n=7) and model group(n=88).The FSGS rat model was established by tail intravenous injection of adriamycin twice a week for two times and unilateral renal resection.After making a successful model, the rats were randomly divided into model group, losartan group, Shenshu(BL23) moxibustion group(short-term, medium-term and long-term) and Geshu(BL17) moxibustion group(shortterm, medium-term and long-term), with 11 rats in each group.Rats in each group were given corresponding intervention.After 12 weeks, immunohistochemical method was used to detect the protein expression of VEGF, VEGFR-2, ET-1, Ang II and AT1 R in renal tissue; nitrate reductase method was used to measure the content of NO in renal tissue.Results:Compared with the sham operation group, the protein expressions of VEGF, VEGFR-2, ET-1, Ang II and AT1 R in the model group were significantly increased and the contents of NO were significantly decreased(P<0.01); compared with the model group, the expressions of VEGF, VEGFR-2, ET-1, Ang II and AT1 R in losartan group, long-term Shenshu(BL23) moxibustion group and long-term Geshu(BL17) moxibustion group were decreased and the contents of NO were increased(P<0.05, P<0.01).Conclusion:Moxibustion may protect the renal tissue by down-regulating the protein expression of VEGF and its receptor VEGFR-2.