左归丸含药血清对小鼠早期胚胎体外发育的影响

Effects of Zuogui Pill serum on early embryo development in vitro in mice

目的:研究高糖环境下滋阴补肾方药左归丸对小鼠胚胎体外发育的影响。方法:体外培养小鼠胚胎,用高浓度葡萄糖抑制胚胎发育,加左归丸含药血清干预胚胎体外发育过程,用囊胚率,2-细胞率和囊胚细胞计数来评估胚胎发育的情况。结果:高糖环境抑制胚胎发育,选择40mmol/L为高葡萄糖抑制胚胎发育的浓度,与正常组比较,囊胚率下降(P<0.05),用左归丸含药血清干预高糖抑制组,囊胚率升高(P<0.05)。从囊胚细胞计数来看,高糖环境减少囊胚细胞数(P<0.05,P<0.01),左归丸含药血清进一步减少囊胚细胞数,但能有效提高囊胚率。结论:高糖抑制胚胎发育,左归丸含药血清可以提高高糖干预下受抑制的胚胎发育率,促进胚胎发育。

Objective: To study the effects of glucose and Zuogui Pill serum on the in vitro development of mouse embryos in high glucose. Methods: Embryos were cultured in vitro and the embryonic development was inhibited by high level of glucose. Zuogui Pill serum was used to increase the development of embryos. The blastocyst rate, 2-cell rate and blastocyst nucleus count were used to evaluate the embryonic development. Results: High level of glucose inhibited the embryonic development, and 40mmol/L was selected as the concentration of high glucose to inhibit the embryo development. Compared with the control group, the blastocyst rate of high glucose was decreased(P<0.05). Compared with the model group, blastocyst rate of Zuogui Pill serum was increased(P<0.05). From the blastocyst nucleus counting, the high glucose environment reduced the number of blastocyst cells(P<0.05, P<0.01). Zuogui Pill serum further reduced the number of blastocysts, but it could improve the rate of blastocyst. Conclusion: High level of glucose reduces the development of embryo while Zuogui Pill serum promotes the blastocyst rate, and promote embryonic development.