薏苡仁多糖不同组分对脾虚水湿不化大鼠模型免疫功能的影响

Effects of different components of coix seed polysaccharides on the immune function of model rats with syndrome of stagnation of fluid-dampness due to spleen deficiency

目的:研究薏苡仁多糖不同组分对脾虚水湿不化大鼠模型免疫功能的影响。方法:采用高脂低蛋白加力竭游泳法复制脾虚水湿不化大鼠模型,检测模型大鼠体质量、血清蛋白、胸腺指数、脾脏指数、血清IFN-γ、IL-2、IL-4、IgG、IgA、C3水平。结果:给药干预后,与模型对照组比较,粗多糖组和多糖组分Ⅰ组大鼠体质量,血清蛋白,胸腺指数,脾脏指数,血清IFN-γ、IL-2、IgG、IgA、C3含量显著升高(P<0.05,P<0.01),IL-4含量显著降低(P<0.05)。结论:薏苡仁多糖不同组分能改善脾虚水湿不化大鼠免疫功能,其中多糖组分Ⅰ作用显著。其机制可能与恢复Th1/Th2平衡,提高免疫球蛋白等的水平有关。

Objective: To explore the effect of different components of coix seed polysaccharides on the immune function of model rats with syndrome of stagnation of fluid-dampness due to spleen deficiency. Methods: The rat model with syndrome of stagnation of fluid-dampness due to spleen deficiency was induced by high fat and low protein diet plus swimming exhaustion method. The body weights, serum proteins, thymus index, spleen index, and serum contents of IFN-γ, IL-2, IL-4, IgG, IgA and C3 were taken as outcome measures to evaluate the effect of coix seed polysaccharides and components. Results: Compared with model group, body weight, serum protein, thymus index, spleen index, serum level of IFN-γ, IL-2, IgG, IgA and C3 in coix seed polysaccharides group and polysaccharide-Ⅰ group were significantly improved(P<0.05, P<0.01), while IL-4 was decreased significantly(P<0.05). Conclusion: Different components of coix seed polysaccharides could promote the immune function of model rats with syndrome of stagnation of fluid-dampness due to spleen deficiency, and polysaccharide-Ⅰ shows significant effect. Its mechanism may be related to the adjustment of balance between Th1 cell and Th2 cell and the improvement of immunoglobulin level.