金乌健骨方兔含药血清冻干粉对类风湿关节炎滑膜细胞增殖作用的影响

Effects of medicated serum frozen powder of Jinwu Jiangu Formula on synovial cell proliferation in rheumatoid arthritis

目的:探讨苗药金乌健骨方兔含药血清对类风湿关节炎成纤维样滑膜细胞(RA-FLS)增殖的作用。方法:选取符合RA诊断标准的骨科手术患者,取滑膜组织进行滑膜细胞培养,并制备金乌健骨方兔含药血清,用小牛血清调至不同浓度,取3-5代对数生长期滑膜细胞加入不同浓度含药血清冻干粉,采用MTT法检测各组细胞生长抑制情况,流式细胞仪检测凋亡情况。结果:空白对照组RA-FLS持续增殖,应用含药血清冻干粉干预48h后,随着金乌健骨方含药血清浓度的升高,OD值逐渐降低,细胞抑制率逐渐升高。流式细胞检测结果显示金乌健骨方高、中、低剂量组和阳性药对照组细胞凋亡率增加,与空白对照组比较,均具有统计学意义(P<0.01)。结论:苗药金乌健骨方兔含药血清冻干粉具有抑制类风湿关节炎成纤维样滑膜细胞的增殖作用。

Objective:To investigate the medicated serum of Miao medicine Jinwu Jiangu Formula on the proliferation of fibroblast like synoviocytes(RA-FLS) in rheumatoid arthritis.Methods:The patients were selected according to the diagnostic criteria of RA,and the synovial tissue was taken from the patients.Jinwu Jiangu Formula containing serum was prepared in rabbits.Different concentrations of medicated serum frozen powder were added to the 3-5 generation of the logarithmic phase of synovial cells.MTT method was used to detect the cell growth inhibition in each group,and flow cytometry was used to detect apoptosis.Results:RA-FLS in blank control group continued to proliferate,after using medicated serum frozen powder intervention for 48 h,with increasing concentrations of Jinwu Jiangu Formula containing serum,OD value was decreased,and the inhibition rate was increased gradually.Flow cytometry showed that the apoptosis rate increased with Jinwu Jiangu Formula high,medium,low dose groups and positive drug control group.Compared with the blank control group,they all had statistical significance(P<0.01).Conclusion:Medicated serum frozen powder of Miao medicine Jinwu Jiangu Formula can inhibit the proliferation of fibroblast like synoviocytes in rheumatoid arthritis.