基于近红外光谱法对不同商品规格地黄进行定性及定量研究

Qualitative and quantitative study on different commercial specifications of Radix Rehmanniae based on near infrared spectroscopy

目的:建立快速鉴别不同商品规格地黄的定性和定量模型。方法:对不同商品规格的地黄按重量、表面颜色、断面颜色及断面质地进行等级划分,利用高效液相色谱(HPLC)测定环烯醚萜苷类、糖类成分含量,利用判别分析及最小偏二乘法分别建立不同商品规格地黄的定性和定量模型。结果:等级模型中,各个等级之间分离较差,断面颜色模型中,将断面外黄内棕归为棕褐色,所建模型稳定、可靠。定量分析中,校正模型的R^2为0.9831,RMSEC为0.0672,RMSEP为0.278。结论:所建立的定性和定量模型准确、可靠,可快速评价不同商品规格地黄的品质。

Objective: To establish qualitative and quantitative models for the rapid identification of different commercial specifications of Radix Rehmanniae. Methods: According to the weight, the color of the surface, the color and the texture of the cross-section, different commercial specifications of Radix Rehmanniae were classified. The contents of iridoid glycosides andcarbohydrates were determined by HPLC. The qualitative and quantitative models of different commercial specifications of Radix Rehmanniae were constructed by discriminant analysis and least squares method respectively. Results: In the grade model, the separation among the grades was poor. In the cross-section color model, the outer yellow and inner brown of the cross-section was classified as tan, and the model was stable and reliable. In the quantitative analysis, the root-mean-square error of crossvalidation,the root-mean-square error of calibration and the R^2, RMSEC and RMSEP of the correction modelwere 0.9831, 0.0672 and 0.278 respectively. Conclusion: The qualitative and quantitative models established in this study are accurate and reliable, and can be used to quickly evaluate the quality of different commercial specifications of Radix Rehmanniae.