肺纤方提取物对肺间质纤维化大鼠VEGFR1介导的p38MAPK/PI3K信号通路表达的影响

Effects of Feiqian Formula extract on the expression of VEGFR1-mediated p38MAPK/PI3K pathway in rats with pulmonary interstitial fibrosis

目的:探讨肺纤方提取物对肺间质纤维化大鼠血管新生的影响。方法:将大鼠分为假手术组、模型组、泼尼松组、氯沙坦组、肺纤方大、中、小剂量组7组,除假手术组外,采用气管插管灌注博莱霉素3mg/kg进行大鼠肺间质纤维化造模;造模第2天予以药物灌胃干预。于14d及28d分两批进行动物处理,免疫组化SP法检测VEGFR1、p38MAPK、PI3K蛋白表达。结果:肺纤方大剂量组均减轻VEGFR1、p38MAPK、PI3K蛋白表达,较模型组差异显著(P<0.05)。结论:肺纤方通过减少肺泡炎及纤维化修复时异常血管新生介导的胶原沉积而具有抗肺间质纤维化作用。

Objective: To investigate the effects of Feixian Formula extract on angiogenesis in rats with pulmonary interstitial fibrosis. Methods: The rats were divided into sham operation group, model group, Prednisone group, Losartan group, and Feixian Formula high, middle, and low dose groups. The endotracheal intubation was used to make the pulmonary interstitial fibrosis model with 3mg/kg of Bleomycin except the sham operation group. In the next day after modeling, rats were treated with respective drugs by gavage. Samples were collected at 14 d and 28 d, and the expression of VEGFR1, p38 MAPK and PI3 K protein was detected by immunohistochemistry SP method. Results: The expressions of VEGFR1, p38 MAPK and PI3 K protein in Feixian Formula high dose group were significantly lower than those in model group(P<0.05). Conclusion: Feixian Formula exerts antipulmonary fibrosis effects, which is acted by reducing alveolar catarrh and abnormal angiogenesis-mediated collagen deposition in fibrotic reparation.