健脾益智胶囊对大脑中动脉栓塞大鼠海马BDNF、EGF、bFGF表达的影响

Effects of Jianpi Yizhi Capsule on expression of BDNF, EGF and bFGF in the hippocampus of MCAO rats

目的:探讨健脾益智胶囊对大脑中动脉栓塞(MCAO)大鼠海马脑源性神经营养因子(BDNF)、表皮生长因子(EGF)、成纤维细胞生长因子(bFGF)表达的影响。方法:建立MCAO大鼠模型,随机分为正常组、假手术组、模型组、西药组、中药组。采用免疫组化法于术后7、14、28d观察海马BDNF、EGF、bFGF表达情况。结果:术后7d,中药组BDNF、EGF、bFGF表达显著高于模型组、西药组(P<0.05)。术后14d,中药组BDNF、EGF表达显著高于模型组、西药组(P<0.05);bFGF表达与西药组无差异。术后28d,中药组BDNF、bFGF表达显著高于模型组(P<0.05),与西药组无差异;EGF表达无差异。结论:健脾益智胶囊可促进BDNF、EGF、bFGF阳性细胞数量增加,提示健脾益智胶囊能可能通过诱导BDNF、EGF、bFGF以促进缺血后神经干细胞增殖分化。

Objective: To study the effects of Jianpi Yizhi Capsule on expression of BDNF, EGF and b FGF in the hippocampus of middle cerebral artery occlusion(MCAO) rats. Methods: MCAO model rats were built, and were randomly divided into 5 groups: normal control group, sham operation group, MCAO group, nimodipine group, Jianpi Yizhi Capsule group. 7, 14 and 28 days after the operation, the expression of BDNF, EGF and b FGF in the hippocampus were assayed respectively by immunohistochemistry. Results: The expression levels of BDNF, EGF and b FGF on the 7th day of the Jianpi Yizhi Capsule group were significantly higher than MCAO and nimodipine group(P<0.05). On the 14 th day, The expression levels of BDNF, EGF of the Jianpi Yizhi Capsule group were significantly higher than MCAO and nimodipine group(P<0.05), while there was no difference comparing with the nimodipine group in the expression of b FGF. The expression levels of BDNF and b FGF on the 28 th day of the Jianpi Yizhi Capsule group were significantly higher than MCAO group(P<0.05), but there was no difference comparing with nimodipine group; the expression of EGF had no difference between the three groups. Conclusion: Jianpi Yizhi Capsule can increase the BDNF, EGF and b FGF positive cells, which suggests that the Jianpi Yizhi Capsule may active the BDNF, EGF and b FGF to regulate the proliferation and differentiation of neural stem cells in the dentate gyrus of hippocampus after ischemia.