不同发汗方法玄参中核苷类成分的UPLC-QTRAP-MS/MS分析

UPLC-QTRAP-MS/MS analytical study on nucleosides and nucleobase of Radix Scrophulariae processed by different watering processing methods

目的:建立超高效液相色谱-串联四级杆/线性离子阱质谱(UPLC-QTRAP-MS/MS)同时测定玄参中10种核苷类成分含量的方法,并分析不同发汗方法玄参中核苷类成分的含量差异。方法:采用UPLC-QTRAP-MS/MS技术同时测定玄参样品中10种核苷类成分的含量。结果:玄参核苷类成分中,以鸟苷、尿苷、腺苷、尿嘧啶含量较高;不同发汗方法玄参中核苷含量差异较大,其中,《中华人民共和国药典》加工方法玄参中核苷含量相对较高。结论:该方法准确、可靠,为玄参药材内在质量的综合评价提供新的方法参考。

Objective: To establish an UPLC-QTRAP-MS/MS method for the determination of 10 kinds of nucleosides and nucleobases in Radix Scrophulariae and to investigate the content difference of nucleosides and nucleobases in Radix Scrophulariae processed by different watering processing methods. Methods: UPLC-QTRAP-MS/MS was applied for the analysis of 10 kinds of nucleosides and nucleobases in Radix Scrophulariae. Results: Compared with other nucleosides and nucleobases in Radix Scrophulariae, the content of guanosine, uridine, adenosine and uracil were relatively higher. Differences were found in the contents of nucleosides and nucleobases among Radix Scrophulariae processed by different watering processing methods. The contents were relatively higher in those processed according to Chinese Pharmacopoeia. Conclusion: The established method is accurate and reliable, and provides a scientific basis for the quality evaluation of Radix Scrophulariae