五参汤对心肌梗死后心力衰竭大鼠心房颤动的影响及作用机制初探

Effects of Wushen Decoction on atrial fibrillation in rats with heart failure after myocardial infarction

目的:探讨五参汤对心肌梗死后心力衰竭大鼠心房颤动的影响及作用机制。方法:24只心肌梗死后心力衰竭大鼠随机分为心衰组和五参汤组;10只假手术大鼠归为假手术组。五参汤组大鼠予五参汤治疗,其余2组给予等量0.9%氯化钠溶液。4周后检测大鼠心功能和左房内径,观察心房颤动诱发情况,检测心房纤维化程度及Cx43、PI3k、Akt、Nrf2蛋白表达情况。结果:五参汤可显著抑制房颤诱发及持续时间(P<0.01),改善大鼠心功能并抑制心房扩大、心房纤维化(P<0.05),上调Cx43和PI3k/Akt/Nrf2信号通路(P<0.05)。结论:五参汤显著抑制心肌梗死后心力衰竭大鼠心房颤动的诱发,其作用机制与其抑制心房纤维化和心房缝隙连接重构,上调PI3k/Akt/Nrf2信号通路有关。

Objective: To investigate the effects and mechanism of Wushen Decoction(WST) on atrial fibrillation(AF) in rats with heart failure(HF) after myocardial infarction(MI). Methods: Twenty-four HF rats with HF were randomly divided into HF group(HF group) and Wushen Decoction group(WST group)(n=12, each group). Ten sham operation controlrats were grouped into Sham group. The WST group was treated with Wushen Decoction, and the other groups were administrated with the normal saline. After four weeks intervention, echocardiography, AF vulnerability, atrial fibrosis, connexin 43, PI3 k, Akt, Nrf2 protein expression were assessed. Results: WST resulted in an attenuation of arrhythmogenic left atrial remodeling, with a significant reduction in left atrial diameter(P<0.01), AF inducibility and duration, and atrial fibrosis(P<0.05), and an increase of protein expression of Cx43 and up-regulation of PI3k/Akt/Nrf2 signaling pathway(P<0.05). Conclusion: WST has the potential to reduce arrhythmogenic atrial remodeling and AF vulnerability via anti-fibrosis, inhibiting gap junction remodeling by upregulating PI3k/Akt/Nrf2 signaling pathway.