基于网络药理学方法对两组对药作用机制的对比研究

Comparative study on the mechanism of two groups of drugs based on network pharmacology

目的:以麻黄桂枝和金银花连翘这两组对药为例,探索辛温药和寒凉药现代药理作用机制的异同,为临床更好的使用这两类中药提供数据支持。方法:基于麻黄、桂枝、金银花、连翘相对应的分子-靶标数据,分析得出辛温药和寒凉药共有的作用靶标(HWT)、辛温药的特异性靶标(XWTH)和寒凉药的特异性靶标(HLTH)。再分别对相应靶标进行通路富集分析,从而明确其作用机制的异同。结果:HWT相关通路涉及神经、内分泌、炎性免疫、代谢等方面;XWTH相关通路主要涉及机体的各种合成代谢功能,如氨基酸合成代谢、碳代谢等;而HLTH相关通路主要涉及机体炎性免疫功能。结论:辛温药和寒凉药都能从神经-内分泌-免疫、代谢等方面参与人体防御病原体入侵的过程。辛温药侧重通过影响人体物质合成代谢参与机体防御功能,而寒凉药则直接调节人体的炎性免疫反应。

Objective: To explore the similarities and differences of pharmacokinetic mechanism between pungent-warm herbs(PWH) and cold-cool herbs(CCH) taking ephedra herba/Cinnamomi Ramulus and Flos Lonicerae Japonicae/Forsythiae Fructus for example, and to provide evidence for the better clinical use of these two kinds of traditional Chinese medicine. Methods: Based on the molecular-target data of ephedra herba, Cinnamomi Ramulus, Flos Lonicerae Japonicae and Forsythiae Fructus, we got the same targets between PWH and CCH(HWT), the specific targets of PWH(XWTH) and the specific targets of CCH(HLTH). Then the KEGG pathway analysis of corresponding targets was done to clarify the similarities and differences mechanism between PWH and CCH. Results: The target-related pathways of HWT were involving neuroendocrine, inflammation immunity, metabolism and so on. The pathways of XWTH were involvingvarious metabolic functions, such as amino acid metabolism, carbon metabolism and so on. And the pathways of HLTH were mainly involving inflammation and immune function. Conclusion: Both PWH and CPH could participate in human defense against pathogen invasion through neuroendocrine, inflammation immunity, metabolism and so on. But PWH affect the material metabolism of human body much more, and CPH are directly regulate the body's inflammation and immune.