平消胶囊的抗肿瘤作用及其机制研究

Study on anti-tumor effect and mechanism of Pingxiao Capsules

目的:观察平消胶囊的抗肿瘤作用及其初步的作用机制。方法:提取平消胶囊活性部位,用于体外抗肿瘤细胞增殖和诱导肿瘤细胞凋亡;灌胃给予平消胶囊,采用4种人体肿瘤裸鼠移植瘤模型观察体内抑瘤作用及初步毒性反应。结果:平消胶囊提取物对10种人体肿瘤细胞具有一定的体外增殖抑制作用,体外作用72h,其IC50值在400-900μg/m L之间。平消胶囊连续经口灌胃给药14d,对人乳腺癌MDA-MB-231、人甲状腺癌SW579、人肠癌colo205、人胰腺癌PANC-1的最大抑瘤率分别达到59.00%、53.13%、49.51%、52.14%,且对动物体质量和脏器没有明显影响。平消胶囊提取物能诱导人乳腺癌细胞MDA-MB-231和人甲状腺癌细胞SW579凋亡。结论:平消胶囊对人乳腺癌、甲状腺癌、肠癌、胰腺癌的裸鼠移植瘤生长有明显的抑制作用,量效关系明显,且对主要脏器无明显影响,其机制之一为诱导肿瘤细胞的凋亡。

Objective: To investigate the anti-tumor effect and its mechanism of Pingxiao Capsules. Methods: The active fraction of Pingxiao Capsules was extracted for anti tumor cells proliferation and inducing tumor cells apoptosis in vitro. After the intragastric administration of Pingxiao Capsules, 4 kinds of nude mice models of human tumor were used to observe the anti-tumor effect and toxicity in vivo. Results: The extract of Pingxiao Capsules could inhibit the proliferation of 10 kinds of human tumor cells to some extent in vitro. After 72 hours in vitro, the IC50 were between 400-900μg/m L. After intragastric administration of Pingxiao Capsules for 14 days, the maximum inhibitory rate of breast cancer MDA-MB-231, human thyroid carcinoma SW579, human colon cancer Colo205 and human pancreatic cancer PANC-1 reached 59.00%, 53.13%, 49.51% and 52.14% respectively. Meanwhile, there were no significant effects on body weight and viscera of animals. Conclusion: The results show that Pingxiao Capsules could inhibit the growth of human breast cancer, thyroid cancer, colon cancer and pancreatic cancer in nude mice, and the dose effect relationship was obvious. There is no obvious influence on the main organs, and one of the mechanisms is to induce apoptosis of tumor cells.