三伏灸不同灸量对支气管哮喘新西兰兔细支气管形态的影响

Effects of dog days moxibustion with different dosageon bronchial morphology in bronchial asthmatic New Zealand rabbits

目的:观察三伏灸治疗过程中不同灸量对支气管哮喘(BA)新西兰兔的胸部温度及肺细支气管组织形态结构的影响。方法:选取新西兰雄性兔子30只,随机分为5组:模型(A)组、空白(B)组、对照(C)组、实验(D)组、地塞米松(E)组。进行BA气道重塑模型造模后,于"三伏天"以隔姜灸加中药贴敷法干预,并使用红外热象仪进行胸部温度监测。疗程结束后,取新西兰兔右肺,HE染色观察对比不同组新西兰兔右肺细支气管形态结构的变化情况。结果:干预后,其余4组细支气管内管壁及平滑肌厚度较B组厚(P<0.05);A组与C组支气管内管壁及平滑肌厚度无明显差异;A组细支气管内管壁及平滑肌厚度较D、E组厚(P<0.05)。D组的胸腔体表温度明显高于灸前(P<0.05);C组的胸腔体表温度与灸前无明显差异;D组温度变化幅度明显大于C组(P<0.05);胸腔体表温度随着隔姜灸壮数的升高而升高,Spearman相关系数=0.168,P=0.001,差异具有统计学意义。结论:隔姜灸达到9壮能使BA型新西兰兔细支气管形态结构发生良性变化,从而改善BA型新西兰兔气道重塑的症状,有效缓解BA的症状。胸腔体表温度与隔姜灸的量呈正相关,说明胸腔温度的提升可能与疗效有关,温度的提升可能是隔姜灸时达到"气至病所"的相关指标之一。

Objective: To observe the effect of different dosage of dog days moxibustion on thoracic temperature and bronchial morphology of lung tissues in asthmatic New Zealand rabbits. Methods: Thirty male New Zealand white rabbits were randomly divided into five groups: model(A) group, blank(B) group, control(C) group, experimental(D) group and dexamethasone(E) group. Except the B group, the rest of the groups were modeled by bronchial asthma airway remodeling method. The D group and E group were given moxibustion on ginger and Chinese medicine application therapy, and infrared thermal imager was used to monitor the chest temperature. After the experiment, the right lung of the rabbits was taken and HE staining was used to observe the bronchioles morphology changes. Results: After the intervention, the thickness of bronchial smooth muscle and bronchial wall in the A, C, D and E group were significantly thicker than that in the B group(P<0.05). The thickness of bronchial smooth muscle and bronchial wall in the A group and C group had no significant difference. The thickness of bronchial smooth muscle and bronchial wall in the A group were significantly thicker than those in the D and E group(P<0.05). The pleural surface temperature of the D group after moxibustion was significantly higher than that before moxibustion(P<0.05). The pleural surface temperature of the C group had no significant difference between after moxibustion and before moxibustion. After moxibustion, the change of surface temperature of the D group was significantly higher than that of the C group(P<0.05). The pleural surface temperature of the D group was increased with the moxibustion on ginger of moxa cones(the Spearman correlation coefficient=0.168, P=0.001). Conclusion: Ginger moxibustion reached 9 strong can improve positive changes of bronchial asthma type rabbit bronchial morphological structure, so it can improve the bronchial asthma symptoms of airway remodeling in New Zealand rabbits and alleviate the symptoms of bronchial asthma effectively. At the same time, there is a positive correlation between the body surface temperature and the amount of ginger moxibustion, which indicates that the improvement of the thoracic temperature may be related to the curative effect.