中、韩不同产地艾叶的质量评价及其道地性考察

Quality evaluation and genuine regional analysis on Artemisiae Argyi Folium from different places of China and Korea

目的:通过中、韩不同产地艾叶样品中有效物质挥发油、总黄酮和鞣质的含量测定及出绒率检测,比较中药艾叶的质量,评估"蕲艾"(产于湖北蕲春县一带的艾叶)的道地性。方法:采用紫外-可见分光光度法,以芹菜素为对照品,研究测定艾叶总黄酮含量;采用《中华人民共和国药典》(2015年版)方法测定艾叶挥发油和鞣质含量;采用自拟的方法检测艾叶出绒率。结果:不同产地艾叶中挥发油、总黄酮、鞣质含量均存在一定的差异;蕲艾中挥发油、总黄酮、鞣质的平均含量及平均出绒率明显高于中、韩其他产地艾叶样品的平均值。结论:实验数据支持蕲艾的道地性;同时,所分析的样品中,华东地区江苏、浙江省的艾叶质量也较好。

Objective: To compare Artemisiae Argyi Folium quality and study genuine regional nature of ‘Qi Ai’(from Qichun County, Hubei Province) through determining the contents of bioactive substances volatile oil, total flavonoids and tannins in Artemisiae Argyi Folium samples collected at the same time and from different places of China and Korea and detecting their output rates of moxa. Methods: The content of the total flavonoids was determined by UV-VIS spectrophotometry with apigenin as the reference substance. The contents of volatile oil and tannins were determined by the methods of Pharmacopoeia of the People’s Republic of China(2015). The output rates of mox were detected using our proposed method. Results: The results showed that there were some differences in contents of the volatile oil, total flavonoids and tannins of Artemisiae Argyi Folium from different place of production. Their average contents and average output rates of moxa in the ‘Qi Ai’ samples were obviously higher than those of the samples from the other places. Conclusion: Experimental data support ‘Qi Ai’ as genuine regional medicinal material. Besides, the qualities of the samples from Jiangsu and Zhejiang provinces in East China are also better.