右归饮对腺嘌呤所致肾虚且免疫低下大鼠的改善作用

Improvement of Yougui Decoction on rats with adenine-induced kidney deficiency and immunodeficiency

目的:研究右归饮对腺嘌呤致肾虚且免疫功能低下模型大鼠的改善作用。方法:将正常SD大鼠随机分为正常组(10只)与造模组(20只),正常组灌胃0.5%的羧甲基纤维素钠溶液,造模组用腺嘌呤150mg/kg灌胃,连续56d后再随机分为模型组(10只)、右归饮组(10只),自第57天开始,模型组和右归饮组每隔1天上午给予150mg/kg腺嘌呤灌胃维持模型42d,同时右归饮组于每日下午灌胃20g/kg右归饮,正常组与模型组灌胃等体积的0.9%氯化钠溶液、连续42d。检测大鼠血常规、血清生化,腺体激素水平、免疫分子含量,测定大鼠NK细胞杀伤率及脾淋巴细胞增殖能力。结果:与正常组比较,模型组大鼠血清中肌酐(CREA)、尿素(UREA)及免疫球蛋白M(IgM)含量均显著升高(P<0.01,P<0.05);补体C3含量、外周血中白细胞数目、淋巴细胞数目及淋巴细胞百分比、甲状腺素(T4)、三碘甲状腺原氨酸(T3)、皮质酮(CORT)、睾酮(T)、促肾上腺皮质激素(ACTH)的水平显著下降(P<0.05);NK细胞杀伤率及脾淋巴细胞增殖能力显著下降(P<0.01,P<0.05)。与模型组比较,右归饮组大鼠血清中CREA和UREA含量显著降低(P<0.05);外周血中白细胞数目、淋巴细胞数目,血清中CORT、T、T4含量、补体C3含量、NK细胞杀伤率及脾淋巴细胞增殖能力均显著升高(P<0.05)。结论:右归饮能够通过调节大鼠肾功能及内分泌功能改善肾虚,同时,也能通过调节IgM、C3及免疫细胞数目和功能改善肾虚引起的免疫功能的减退。

Objective:To study the improvement of Yougui Decoction on rats with adenine-induced kidney deficiency and immunodeficiency.Methods:Normal SD rats were randomly divided into control group(n=10)and model group(n=20).The control group was given with 0.5%sodium carboxymethyl cellulose solution by gavage.The model group was given 150 mg/kg adenine by gavage for 56 days and then randomly divided into model group(n=10)and Yougui Decoction group(n=10).Since the 57 th day,the model group and Yougui Decoction group were given 150 mg/kg adenine every other morning to maintain the model for 42 days.At the same time,the Yougui Decoction group was given 20 g/kg Yougui Decoction every afternoon,and the control group and the model group were given 0.9%sodium chloride solution of equal volume for 42 days.Blood routine,serum biochemical,thyroid,adrenal,gonadal hormone levels,immune molecules,spleen lymphocyte proliferation ability and NK cell killing rate were measured.Results:Serum creatinine(CREA),urea(UREA)and immunoglobulin M(IgM)levels in the model group were significantly increased than those in the normal group(P<0.01,P<0.05);complement C3,peripheral Number of white blood cells,lymphocyte count and lymphocyte percentage,T4,T3,CORT,T,ACTH,spleen lymph The cell proliferation ability and NK cell killing rate decreased significantly(P<0.05),indicating successful modeling.Compared with the model group,the serum levels of CREA and UREA in the rats of the Youguiyin group were significantly decreased(P<0.05).The serum levels of CORT,T,T4,C3,and the number of white blood cells and lymphocytes in the peripheral blood were significantly increased(P<0.05),rat spleen lymphocyte proliferation ability and NK cell killing rate increased significantly(P<0.05).Conclusion:Yougui Decoction could regulate renal function and endocrine function of rats by changing renal insufficiency,and at the same time,it could also improve the immune function decline caused by kidney deficiency by regulating the number of IgM,C3 and immune cells.