淫羊藿提取物对绝经后骨质疏松症大鼠PGE2及β2肾上腺素受体的影响

Effects of Herba Epimedii extract on PGE2 and β2-adrenergic receptor in postmenopausal rats with osteoporosis

目的:从预防骨丢失和促进骨再生两方面,研究中药淫羊藿提取物对绝经后骨质疏松症的防治作用。方法:通过去卵巢复制绝经后骨质疏松症模型;用淫羊藿提取物、强骨胶囊和己烯雌酚分别灌胃作用模型大鼠90d,观察比较各组骨密度(BMD g/cm3)、骨小梁体积分数(BV/TV)、子宫重量/体质量(UW/BW),骨组织形态计量学指标、前列腺素E2(PGE2)和股骨β2肾上腺素能受体(ADRβ-2R)的表达。结果:与模型组比较,淫羊藿提取物能从骨组织形态学上有效防治绝经后骨质疏松症模型大鼠骨质疏松症,也能明显降低绝经后骨质疏松症模型大鼠血清PGE2水平(P<0.01)。同时,股骨头切片免疫组化分析显示:中、低剂量组淫羊藿提取物能显著降低ADRβ-2R的表达(P<0.05)。结论:淫羊藿提取物具有抑制骨吸收、促进骨形成的作用,其防治骨质疏松的作用机制涉及PGE2/ADRβ-2R信号通路。

Objective:From the aspects of preventing bone loss and promoting bone regeneration,to research the prevention and treatment of Herba Epimedii extract of Chinese medicine on postmenopausal osteoporosis,and the signaling pathway of osteogenic effect in TCM theory,to enrich the mechanism of anti-osteoporosis and provide experimental basis.Methods:Postmenopausal osteoporosis model was established through the ovariectomy;the model rats were intragastrically administered Herba Epimedii extract,Qianggu Capsule and diethylstilbestrol respectively for 90 days,and the bones of each group,density(BMD g/cm3),trabecular bone volume/tissue volume(BV/TV),uterine weight/body weight(UW/BW),bone histomorphometry,expression of the prostaglandin E2(PGE2)andβ2 adrenergic receptor(ADRβ-2 R)were observed and compared.Results:Compared with the model group,Herba Epimedii extract can effectively prevent osteoporosis in postmenopausal osteoporosis model rats from bone morphology.The serum PGE2 level in postmenopausal osteoporosis model rats was decreased(P<0.01).At the same time,immunohistochemical analysis of caput femoris sections showed that Herba Epimedii extract significantly reduced the expression of ADRβ-2 R(P<0.05).Thereby leading to a decrease in the secretion of osteoclast differentiation factors.Conclusion:Herba Epimedii Extract inhibit bone resorption,promoting bone formation,and its mechanism relates to prevention and treatment of osteoporosis PGE2/ADRβ-2 R signaling pathway.This provides experimental basis for the prevention and treatment of osteoporosis with the theory of‘kidney holds bone’in Chinese medicine.