红芪多糖对db/db小鼠糖尿病心肌病心肌组织氧化应激的影响

Effects of hedysarum polybotys saccharide on controlling oxidative stress in myocardial tissue of diabetic cardiomyopathy in db/db mice

目的:研究红芪多糖(HPS)对调控db/db小鼠糖尿病心肌病(DCM)心肌组织中抗氧化基因表达的影响。方法:60只db/db小鼠随机平均分为5组:对照组,模型组,红芪多糖(HPS)高、中、低剂量组;正常组为12只同周龄同背景的雄性db/m小鼠。连续灌胃干预8周。检测血脂及心肌组织中丙二醛(MDA)含量;蛋白印记法和反转录聚合酶链式反应法检测小鼠心肌组织Nrf2以及其下游血红素加氧酶-1(HO-1)、谷胱甘肽还原酶(GR)、氨酰半胱氨酸合成酶催化亚单位(GCLC)mRNA和蛋白的表达。结果:药物治疗8周后,与模型组比较,各给药组的MDA含量均显著下降(P<0.01,P<0.05);HPS高、中剂量组及对照组Nrf2、HO-1蛋白表达显著升高(P<0.01),各给药组GCLC、GR蛋白表达均显著升高(P<0.01,P<0.05);HPS高、中剂量组及对照组Nrf2、GCLCmRNA表达显著升高(P<0.01),各给药组HO-1、GRmRNA表达均显著升高(P<0.01)。结论:HPS可能通过增加Nrf2的表达影响抗氧化酶类的表达,从而调控氧化应激改善db/db小鼠DCM心肌损伤。

Objective:To study the effects of Hedysarum polybotys saccharide(HPS)on the expression of antioxidant genes in myocardial tissue of diabetic cardiomyopathy in db/db mice.Methods:According to the weight,60 db/db mice were randomly divided into 5 groups:Hedysarum polybotys saccharide high,medium and low dose experimental group,control group,model group;normal group were 12 male db/m mice of the same age and background.They were continuous intragastric intervention for 8 weeks.Detection of blood lipids and malondialdehyde in myocardial tissue(MDA)content;protein imprinting and reverse transcription polymerase chain reaction assay for detection the expression of Nrf2 and protein in the downstream such as HO-1,GR,GCLC mRNA in mouse myocardial tissue.Results:After 8-week treatment,compared with the model group,MDA content in each drug intervention group decreased significantly(P<0.01,P<0.05).The expression of Nrf2 and HO-1 protein in the HPS high and medium dose groups and the control group were significantly increased(P<0.01),and the expressions GCLC and GR protein in each drug intervention group were significantly increased(P<0.01,P<0.05),the expressions of Nrf2 and GCLC mRNA in the HPS high and medium dose groups and the control group were significantly increased(P<0.01),and the expressions of HO-1 and GR mRNA in each drug intervention group were significantly increased(P<0.01).Conclusion:HPS may improve the regulation of oxidative stress in db/db mice myocardial DCM by increasing the expression of Nrf2 to affect the expression of antioxidant enzyme.