野芭蕉多糖对自发性高血压大鼠血管平滑肌细胞增殖的影响及其与胆固醇调节元件结合蛋白表达的关系

Effects of Musa balbisiana polysaccharide on the proliferation of vascular smooth muscle cells in SHR rats and their relationship with sterol regulating elements binding protein expression

目的:探讨野芭蕉多糖对自发性高血压大鼠(SHR)血管平滑肌细胞(VSMC)增殖的抑制作用及其与胆固醇调节元件结合蛋白(SREBP)的关系。方法:分离SHR VSMC传代培养取得VSMC系后,分成4组:高、中、低浓度组(0.01、0.1、1.0μmol/L野芭蕉多糖)和对照组(纯化水),用第4代VSMC进行药物干预培养,于干预培养24、48、100、120h,分别检测VSMC细胞活力及增殖周期,取干预培养100h的标本检测SREBP mRNA相对表达量,并与对照组比较。结果:与对照组比较,中、高浓度野芭蕉多糖组VSMC细胞增殖率显著降低(P<0.01),增殖周期(G0+G1期)显著延长(P<0.01),SREBP mRNA相对表达量受到显著抑制(P<0.01)。药理效应与剂量呈正相关关系。结论:野芭蕉多糖可有效抑制SHR VSMC的过度增殖,其作用机制可能与下调SREBP mRNA表达、调节脂质代谢紊乱有关。

Objective:To study the inhibitory effect of Musa balbisiana polysaccharide on proliferation of vascular smooth muscle cells(VSMC)in SHR rats and their relationship with sterol regulating element binding protein(SREBP).Methods:Separation collaterals disease rat VSMC of subculture,VSMC medium could be divided into four groups:high-dose,mediumdose and low-dose groups and control group giving,the concentration of 0.01,0.1,1.0μmol/L and water for injection,with the fourth generation of VSMC drug intervention,the intervention training 24 h,48 h,100 h and 120 h specimen,VSMC proliferation rate was detected in proliferation cycle,take intervention training samples sterol regulation of 100 h element binding protein SREBP mRNA expression of relative quantity,and compared with the control group.Results:Compared with the control group,the proliferation rate of VSMC cells in the medium-dose,high-dose,group decrease(P<0.01),and the proliferation cycle(G0+G1)was prolonged(P<0.01),and the relative expression of SREBP mRNA was inhibited(P<0.01).The pharmacological effect and the dose relationship.Conclusion:Musa balbisiana polysaccharide can effectively inhibit the excessive proliferation of VSMC in the rats,and its mechanism may be related to down-regulation of SREBP mRNA expression and regulation of lipid metabolism disorder.