马拉硫磷对大鼠睾丸标志酶活力和精子质量的影响

Effects of malathion on testicular enzyme activities and sperm quality of male rats

目的探讨马拉硫磷亚慢性染毒对雄性大鼠睾丸标志酶活力和精子质量的影响。方法40只SPF级未成年Wistar雄性大鼠被随机分为对照组和3个染毒组。染毒组分别以33．75、54．00、108．00mg／kg（1／32LD50、1／20LD50,1／10LD50）马拉硫磷对雄性大鼠连续灌胃染毒60d，对照组给予等量蒸馏水。染毒后，测定大鼠体重，处死后立即取睾丸和附睾并测定其重量；取左侧附睾尾测定大鼠精子活率、精子数量及精子畸形率；左侧睾丸冻存于-80℃测定睾丸标志酶酸性磷酸酶（ACP）、乳酸脱氢酶（LDH）、山梨醇脱氢酶（SDH）、γ-谷氨酰转肽酶（γ-GT）活力。结果54．00、108．00mg／kg染毒组大鼠体重、睾丸重呈下降趋势，与对照组的差异有统计学意义（P〈0．05），染毒组大鼠精子数量及精子活率降低，精子畸形率上升，108．00mg／kg染毒组与对照组的差异有统计学意义（P〈0．05）。108．00mg／kg染毒组ACP和γ—GT活力降低，LDH活力上升，与对照组的差异均有统计学意义（P〈0．05）；SDH活力未见明显改变。结论马拉硫磷降低了雄性大鼠精子数量和精子活率，提高了精子畸形率，抑制了大鼠睾丸标志酶活力。

Objective The aim of this study is to investigate the effects of sub chronic exposure to malathion on testieular enzyme activities and sperm quality of male rats. Methods Forty male rats were divided into four groups： three exposure groups and a control group. Malathion was administered orally to male rats at 0, 33.75, 54.00, and 108.00 mg/kg for 60 days to evaluate the toxic alterations in sperm dynamics and testicular enzyme activities including ACP,LDH,SDH and γ-GT. The control rats were administered with an equivalent volume of distilled water in the same manner.After sacrificed ,the testes were collected and weighed. Results The body weight and the testis weight of animals showed a decreasing tendency, and there was a statistical difference between the 54.00, 108.00 mg/kg groups, and the control group （P〈0.05）. Malathion brought about marked reduction in testicular sperm counts, sperm motility, and significant growth of sperm malformation rate in 108.00 mg/kg group. A significant decrease in the activities of testicular enzyme ACP and γ-GT was observed in malathion exposed rats, while the activities of LDH was significant increased and there were no obvious effects on the activities of SDH. The activities of ACP, γ-GT and LDH showed a statistical difference between the 108.00 mg/kg groups, and the control group.Conclusion Malathion reduced the sperm counts and sperm motility, increased the malformation rate and reduced the activities of testicular enzymies of male rats.