摘要
目的:为使分离自具有民族特色和特殊功效的内蒙古牧区马奶及其制品中的乳酸菌更好地应用于发酵工业,对乳酸菌进行耐药性实验并研究其中是否存在由主动外排蛋白介导的喹诺酮类耐药机制。方法:采用二倍稀释法测定乳酸菌对临床常用喹诺酮类药物的最小抑菌浓度(minimum inhibitory concentration,MIC),利用质子泵抑制剂(利血平)结合荧光分光光度法检测耐药菌株和对照菌株对环丙沙星的摄取情况。结果:菌株HZ18、HZ28、NN3、NN4具有耐药性并在加入利血平后菌体内环丙沙星累积量明显增加。结论:乳酸菌中存在主动外排蛋白介导的喹诺酮类耐药机制。
Purpose： In this study, lactic acid bacteria（LAB） were separated from horse milk and horse milk products with national characteristics and special functions in pastoral areas of Inner Mongolia. In order to ensure better application of the isolated strains in the fermentation industry, it is necessary to test their drug resistance and explore if LAB possess an active efflux system mediating quinolone resistance. Methods： Double dilution method was used to determine the minimum inhibitory concentration（MIC） of quinolone. The proton pump inhibitor reserpine was combined with fluorescence spectrophotometry to detect the ciprofloxacin intakes of drug-resistant strains and control strains. Results： The accumulation of ciprofloxacin increased significantly in drug-resistant strains HZ18, HZ28, NN3 and NN4 after adding reserpine. Conclusion： Lactic acid bacteria may possess an active efflux system mediating quinolone resistance.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第9期150-154,共5页
Food Science
基金
国家自然科学基金地区科学基金项目(31060014)
内蒙古自治区高等学校科学研究项目(NJ09059)
关键词
乳酸菌
喹诺酮类药物
最小抑菌浓度
荧光分光光度法
主动外排
lactic acid bacteria
quinolone
minimum inhibitory concentration（MIC）
fluorescence spectrophotometry
active efflux system
