副猪嗜血杆菌的分离鉴定及药敏试验

Isolation,Identification and Antibiotic Susceptibility Test of Haemophilus Parasuis

该研究从广东中山某猪场疑似患有副猪嗜血杆菌(HPS)病的病死猪心脏和肺脏中分离到一株细菌。经形态学观察、培养特性鉴定、生化试验、PCR检测和致病性试验,显示该菌为HPS。药敏试验结果表明该菌株对恩诺沙星、诺氟沙星、红霉素、庆大霉素和新霉素等表现高敏感性;对阿米卡星、土霉素、青霉素、强力霉素和四环素等则耐药。

in this study,a strain of bacteria was isolated from the heart and lung of dead pig which were suspected to be infected with Haemophilus parasuis （HPS） of swine in Zhong- shan, Guangdong province. The 1 strain is identified based on morphologic, cultural, biochemical characteristics,PCR detection and animal pathogenicity test of HPS. Drug sensitive test showed that the HPS was highly sensitive to enrofloxacin,norfloxacin,erythromycin,gentamicin and neomycin,but was strong drug resistant to amikacin,oxytetracycline,penicillin,doxycycline and tetracycline.