缅甸蟒养殖种群的遗传多样性分析

Analysis on Genetic Diversity in Breeding Populations of Python bivittatus

利用微卫星标记分析缅甸蟒养殖群体的遗传多样性,结果表明:在42个缅甸蟒个体中,共检测到76个等位基因,平均有效等位基因数为5.78个,平均期望杂合度为0.815,平均多态信息含量为0.78,8个微卫星位点呈现高度多态性;仅有3个位点显著偏离了Hardy-Weinberg平衡;缅甸蟒海南养殖群体表现出较高的遗传多样性水平。突变-漂移平衡分析结果表明,群体部分位点杂合显著过剩,并未显著偏离突变-漂移平衡,近期没有经历过瓶颈效应,群体数量无明显下降。

Genetic diversity of breeding populations of Burmese python （Python bivittatus） were investigated using microsatellite DNA loci. The study showed that 76 alleles were detected among 42 sampled individuals, the average number of effective allele was 5.78, average of expected heterozygosity was 0. 815, and average of PIC was 0. 78. It was indicated that all the 8 loci were highly polymorphic, and only 3 loci were obviously departured from Hardy-Weinberg equilibrium High level of genetic diversities was represented in breeding popution of Python bivittatus） in Hainan. Mutation-drift equilibrium tests indicated that loci with significantly heterozygote excesses in populations, and the population was not obviously departured from mutation-drift equilibrium. It might indicate that the population did not suffer bottleneck effects in recent time, and no significant decline in the number of populations.