摘要
目的:比较不同方法检测血浆游离氨基酸的准确性。方法应用全自动氨基酸分析仪( Li+系统)测定40例健康志愿者血浆游离氨基酸,与中外文献不同方法健康志愿者氨基酸测定结果进行对比分析。结果本研究氨基酸分析仪( Li+系统)测定40例健康志愿者血浆游离氨基酸结果中,天冬酰胺(Asn)、谷氨酸(Glu)、谷氨酰胺(Gln)、缬氨酸(Val)、酪氨酸(Tyr)、苯丙氨酸( Phe)、鸟氨酸( Orn)、精氨酸( Arg)等主要氨基酸的正常值与国内外氨基酸分析仪测定结果相近(均P>0.05),且与液相色谱-质谱联用法(LC-MS/MS)测定结果较为相近(均P>0.05),除Glu、Tyr外,其他氨基酸测定结果与高效液相色谱法(HPLC)测定结果偏差大(均P<0.05)。结论采用氨基酸分析仪法( Li+系统)和液相色谱-质谱联用法( iTRAQ试剂)检测的结果较为客观和准确,适用于分析血浆游离氨基酸。
Objective To evaluate the accuracy of different methods in detecting free amino acids in plasma.Method 40 blood samples from healthy volunteers were analyzed by an automatic amino acid analyzer ( Li+system) and the results compared with previous reports using other analyzers.Results The results ob-tained by this analyzer for major amino acids [asparagine (Asn), glutamic acid (Glu), glutamine (Gln), valine (Val), tyrosine (Tyr), phenylalanine (Phe), ornithine (Orn), arginine (Arg)] were similar with those previously reported using other amino acid analyzers ( all P〉0.05 ) and liquid chromatography tandem-mass spectrometry (LC-MS/MS) (all P〉0.05).Except for Glu and Tyr, the results for major amino acids showed large deviation compared to the results from high performance liquid chromatography ( HPLC) ( all P〈0.05).Conclusion The amino acid analyzer (Li+) and LC-MS/MS (iTRAQ kit) could accurately detect free amino acids in plasma.
出处
《中华临床营养杂志》
CAS
CSCD
2016年第2期115-120,共6页
Chinese Journal of Clinical Nutrition
关键词
血浆游离氨基酸
氨基酸分析仪
液相色谱-质谱联用仪
健康志愿者
Free amino acids in plasma
Amino acid analyzer
Liquid chromatography tandem-mass spectrometry
Healthy volunteers