少腹逐瘀汤配合针刺对痛经大鼠的镇痛作用及对子宫收缩性和前列腺素水平的影响

Analgesic effect of acupuncture combined with Shaofuzhuyu decoction and its impact on uterine contractility and serum prostaglandin level in dysmenorrhea rats

目的观察少腹逐瘀汤配合针刺对痛经大鼠的镇痛作用和对大鼠子宫收缩性及血清前列腺素(PG)水平的影响,探究其治疗大鼠痛经的机制。方法将80只雌性大鼠随机分为对照组、模型组、药物组、针刺组及药物配合针刺组5组,每组16只。对照组大鼠不进行造模处理,其他4组建立原发性痛经模型,其中药物组、针刺组及药物配合针刺组分别予少腹逐瘀汤、针刺及少腹逐瘀汤联合针刺治疗。以扭体反应评价各组大鼠的疼痛缓解情况,应用BL-420F生物机能系统记录子宫收缩情况,应用ELISA试剂盒检测PGF_(2α)、PGE_2含量。结果与对照组比较,模型组大鼠扭体潜伏期明显缩短,扭体次数增加,子宫收缩波个数显著增加,收缩波峰值显著升高,子宫活动度显著增加(P均<0.01)。与模型组比较,治疗后药物组、针刺组及药物配合针刺组的扭体潜伏期明显延长(P<0.05),扭体次数明显减少(P均<0.01),子宫收缩波个数明显减少,收缩波峰值明显降低,子宫活动度明显降低(P均<0.01),其中药物配合针刺组的上述变化均优于药物组和针刺组(P均<0.01)。与其他各组比较,模型组血清PGF_(2α)的含量最高,PGE_2含量最低(P均<0.01),治疗后,各治疗组PGF_(2α)的含量均有下降,PGE_2含量均有升高(P均<0.01),其中药物配合针刺组的这种变化均优于药物组及针刺组(P均<0.01)。结论少腹逐瘀汤配合针刺治疗可有效缓解大鼠原发性痛经,降低痛经大鼠子宫收缩强度,其机制可能与降低痛经大鼠血清PGF_(2α)含量,增加PGE_2含量有关。

Objective To observe the analgesic effect of acupuncture combined with Shaofuzhuyu decoction and its influence on uterine contractility and serum prostaglandin（ PG） level in dysmenorrhea rats and explore the mechanism for treating primary dysmenorrhea. Methods Eighty female rats were randomized into five groups（ n = 16 each） ： control group,model group,medicine group,acupuncture group and acupuncture plus medicine group. The modeling was not made in control group,and primary dysmenorrheal model was made in other groups in which Shaofuzhuyu decoction,acupuncture,acupuncture plus medicine were respectively used. Pain relief situation of dysmenorrheal rats was assessed by writhing response. Uterine contraction action was recorded with BL-420 F biological function system. Serum PGF_（2α）and PGE_2 levels were detected by ELISA kit. Results Compared with control group,in model group,the latent period of writhing response was significantly shortened; the writhing times significantly increased; the number of uterine contraction wave significantly increased; the peak of systolic wave significantly rose; the uterine activity significantly increased（ all P 0. 01）. Compared with model group,after treatment,the latent period of writhing response was significantly prolonged（ P 0. 05）; the writhing times significantly decreased（ all P 0. 01）; the number of uterine contraction wave significantly decreased; the peak of systolic wave significantly descended; the uterine activity was significantly reduced in medicine group,acupuncture group and acupuncture plus medicine group（ all P 0. 01）; the aforementioned changes after treatment in acupuncture plus medicine group were all superior to medicine group and acupuncture group（ all P 0. 01）. Before treatment,serum PGF_（2α）content in model group was significantly higher than those in remaining groups（ all P 0. 01）,and serum PGE_2 content in model group was significantly lower than those in remaining groups（ all P 0. 01）. After treatment,serum PGF_（2α）content decreased significantly in all treatment group; serum PGE_2 content in all treatment groups increased significantly（ all P 0. 01）; the aforementioned changes after treatment in acupuncture plus medicine group were superior to medicine group and acupuncture group（ all P 0. 01）. Conclusion The treatment of acupuncture combined with Shaofuzhuyu decoction can effectively relieve primary dysmenorrheal of rats,decrease the uterine contraction intensity,and the mechanism may be associated with increasing serum PGF_（2α）level and decreasing serum PGE_2 level.