硫酸化茯苓多糖对帕金森模型鼠脑组织抗氧化能力的影响

Antioxidant effect by sulfated pachymaran in the MPTP mouse model of Parkinson's disease

目的:考察硫酸化茯苓多糖(SP)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的帕金森小鼠脑组织抗氧化能力的影响。方法:MPTP注射ICR小鼠制备帕金森病(PD)模型。将ICR小鼠随机分为5组:对照组、MPTP组和SP治疗组(50,100,150 mg/kg),腹腔注射给药。紫外分光光度法检测小鼠纹状体中超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、乳酸脱氢酶(LDH)的活性和抗超氧阴离子活力以及过氧化氢(H_2O_2)、丙二醛(MDA)的含量,HE染色观察小鼠脑组织形态学变化。结果:与模型组相比,在一定浓度范围内,SP治疗组小鼠纹状体GSH-Px、SOD、CAT、LDH活性及抗超氧阴离子活力有不同程度的升高,H_2O_2和MDA含量有不同程度的下降;海马区神经元排列密集有序,细胞间隙减小,神经元细胞形态结构有不同程度的恢复。结论:SP对MPTP诱导的神经元损伤具有神经保护作用,其潜在机制可能是提高脑组织抗氧化能力,减轻氧化应激反应。

AIM： To investigate the effects of sulfated pachymaran（ SP） on cerebral tissue antioxidant indexes in model mice of Parkinsons ＇ disease（ PD） induced by MPTP. METHODS： ICR mice were treated with MPTP to make PD model. The mice were randomly divided into five groups： control group,MPTP model group and SP group（ 50,100,150 mg / kg）,and all drugs were administrated to mice by abdominal injection. The activity of superoxide dismutase（ SOD）, glutathione peroxidase（ GSH-Px）,catalase（ CAT）,lactate dehydrogenase（ LDH）,anti-superoxide anion and the levels of malondialdehvde（ MDA） and hydrogen peroxide（ H_2O_2） in brain striatum were detected by UV spectrophotometry, and morphologic changes of brain tissue of mice were observed with HE staining.RESULTS： Compared to the model group,treatment of SP in certain concentrations increased the activity of SOD,GSH-Px,CAT,LDH,anti-superoxide anion to varying degree and decreased the levels of H_2O_2 and MDA; intercellular space in the hippocampal region reduced significantly and the neurons became dense and ordered,showing a remarkable recovery from the damage induced by MPTP. CONCLUSION： SP may have a neuroprotective effect on injured neurons induced by MPTP through decreasing oxidative stress reaction.