葛根芩连汤对小鼠肠运动的影响及其配伍分析

Effects of combinations of Gegenqinlian Decoction on small intestine movement in mice and the quantitative analysis

目的:定量分析葛根芩连汤对小肠推进率的配伍规律。方法:运用正交设计方法,用墨汁为显色剂,小鼠小肠推进率为指标(n=80),对其不同配伍进行正交模拟法分析。结果:正常小鼠小肠推进率的基线为73.4%;葛根芩连汤不同配伍降低小肠推进率最优组合是黄连+葛根+炙甘草,理论最大效应达到61.4%(58.7%～64.1%);给药剂量15.21 mL/kg,各组分在复方中的量效关系,黄连最为明显(P<0.01)、葛根和炙甘草较弱,黄芩未呈现;组分间并无明显的相互作用。结论:以降低小肠推进率为主要指标,葛根芩连汤最优组方为黄连、葛根和炙甘草合用,未呈现协同和/或拮抗作用。

AIM： To quantitatively analyze combinations of Gegenqinlian Decoction（ GQD）,consisting of Pueraria lobata（ wild） ohwi（ A）,Coptis chinensis franch（ B）,Scutellaria baicalen-sis Georgi（ C） and Glycyrrhiza uralensis fisch（ D）,on small intestine movement in mice. METHODS：Propelling rate of the small intestine was selected as the index by color ink in 80 mice given the combina-tions of GQD（ 15. 21 mL / kg）,and the orthogonal design was used to assign experimental groups of the combinations. Quantitative analysis was performed by the orthogonal simulation method. RESULTS：The different combinations of components in GQD reduced propelling rate of the small intestine in mice significantly. The best combination reached 61. 4%（ 58. 7%- 64. 1%） by component A,component B and component D,and the contribution to combined effect of each component ranks B〉 A 〉D〉 C. Nointeractions were found among the four components in GOD. CONCLUSION： GQD can reduce movement of the small intestine in mice,and the optimal combination is component A,component B and component D with interaction among them.